Anti-Actin [mAbGEa] Universal Antibody

This universal mouse monoclonal antibody was generated against plant actin ACT1 and recognizes all plant (arabidopsis ACT1, 2, 3, 4, 7, 8, 11, 12), animal (e.g., human muscle and cytoplasmic), fungal, and protist actins.

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

From the laboratory of Richard B. Meagher, PhD, University of Georgia.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Anti-Actin [mAbGEa] Universal Antibody
5mL (supernatant) 4-6 weeks
Regular Price:$345.00
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Product Type: Antibody
Antigen: ArabidopsisActin (ACT1)
Accession ID: P0CJ46
Molecular Weight: 45 kDa
Isotype: IgMk
Clonality: Monoclonal
Clone Name: mAbGEa (5-16 H1C8)
Reactivity: Human, Mouse, Rat, Bovine, Drosophila, Ovine, Plant, Xenopus laevis, Yeast, Zebrafish
Immunogen: Purified Arabidopsisactin (ACT1)
Species Immunized: Mouse
Buffer: Cell culture supernatant
Tested Applications: WB, IF, IHC, ELISA
Storage: -80C
Shipped: Cold Packs

Antibody Reactivity
Actin [mAbGEa] Universal
Actin-1 [mAb45a] ACT1, 3, 4, 12
Actin-11 [mAb2345a] ACT1, 3, 4, 7, 11, 12
Actin-2 [mAb13a] ACT2, 8, 11
Actin-8 [mAbGPa] ACT1, 2, 3, 4, 7, 8, 11, 12

Western Blot Analysis

TOP: Reactivity mAbGEa with human (HsB and HsA1, native; HsG1 and HsA2, recombinant) and plant (At-ACT2, recombinant) actins. The numbers on the Coomassie blue–stained gel indicate the amount of actin protein loaded. BOTTOM: Reactivity of mAbGEa with C. intestinalis cytoplasmic-like actin ACTB1 (CiB) and diverse protist actins. Ac, A. castellanii; Mb, ; Cs, C. scutata. About 1 mg of crude recombinant actin was loaded per lane.

Adapted from: Kandasamy, M.K., et al. (2012) Plant Cell 24, 2041-57.

From the laboratory of Richard B. Meagher, PhD, University of Georgia.
  1. Kandasamy, M.K., Gilliland, L.U., McKinney, E.C., and Meagher, R.B. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell 13, 1541-54.
  2. Kandasamy, M.K., McKinney, E.C., and Meagher, R.B. (1999). The late pollen-specific actins in angiosperms. Plant J 18, 681-91.
  3. Kandasamy, M.K., McKinney, E.C., Roy, E., and Meagher, R.B. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell 24, 2041-57.

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