This universal mouse monoclonal antibody was generated against plant actin ACT1 and recognizes all plant (arabidopsis ACT1, 2, 3, 4, 7, 8, 11, 12), animal (e.g., human muscle and cytoplasmic), fungal, and protist actins.
Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
From the laboratory of Richard B. Meagher, PhD, University of Georgia.
Part of The Investigator's Annexe program.
|Molecular Weight:||45 kDa|
|Clone Name:||mAbGEa (5-16 H1C8)|
|Reactivity:||Human, Mouse, Rat, Bovine, Drosophila, Ovine, Plant, Xenopus laevis, Yeast, Zebrafish|
|Immunogen:||Purified Arabidopsisactin (ACT1)|
|Buffer:||Cell culture supernatant|
|Tested Applications:||WB, IF, IHC, ELISA|
Western Blot Analysis
TOP: Reactivity mAbGEa with human (HsB and HsA1, native; HsG1 and HsA2, recombinant) and plant (At-ACT2, recombinant) actins. The numbers on the Coomassie bluestained gel indicate the amount of actin protein loaded. BOTTOM: Reactivity of mAbGEa with C. intestinalis cytoplasmic-like actin ACTB1 (CiB) and diverse protist actins. Ac, A. castellanii; Mb,
Adapted from: Kandasamy, M.K., et al. (2012) Plant Cell 24, 2041-57.
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