Anti-Actin-1 [mAb45a] Antibody

This mouse monoclonal antibody was generated against plant actin ACT1 and recognizes subclasses 4 and 5 of vegetative plant actins (arabidopsis ACT1, 3, 4, 12).

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

From the laboratory of Richard B. Meagher, PhD, University of Georgia.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Anti-Actin-1 [mAb45a] Antibody
100ug In stock
Regular Price:$410.00
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Product Type: Antibody
Antigen: ArabidopsisActin (ACT1)
Accession ID: P0CJ46
Molecular Weight: 45 kDa
Isotype: IgG1
Clonality: Monoclonal
Clone Name: mAb45a (5-15 H7C5)
Reactivity: Plant actins
Immunogen: Purified Arabidopsisactin (ACT1)
Species Immunized: Mouse
Epitope: Asn79 in reproductive plant actins
Purification Method: Protein G column
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1-2 ug/mL)
Storage: -20C
Shipped: Cold packs

Antibody Reactivity
Actin [mAbGEa] Universal
Actin-1 [mAb45a] ACT1, 3, 4, 12
Actin-11 [mAb2345a] ACT1, 3, 4, 7, 11, 12
Actin-2 [mAb13a] ACT2, 8, 11
Actin-8 [mAbGPa] ACT1, 2, 3, 4, 7, 8, 11, 12

Western Blot Analysis

Adapted from: Kandasamy, M.K., et al. (2001) Plant Cell 13, 1541-54.

From the laboratory of Richard B. Meagher, PhD, University of Georgia.
  1. Kandasamy, M.K., Gilliland, L.U., McKinney, E.C., and Meagher, R.B. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell 13, 1541-54.
  2. Kandasamy, M.K., McKinney, E.C., and Meagher, R.B. (1999). The late pollen-specific actins in angiosperms. Plant J 18, 681-91.
  3. Kandasamy, M.K., McKinney, E.C., Roy, E., and Meagher, R.B. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell 24, 2041-57.

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