This hybridoma line constitutively produces a mouse anti-bat Ig Light chain monoclonal antibody.
The hybridoma was generated by fusion of splenocytes from a BALB/c mouse that had been immunized with big brown bat (Eptisicus fuscus) splenocytes. The resulting hybridomas were plated in methylcellulose and subsequently subcloned in liquid culture.
From the laboratory of William T. Lee, PhD, Health Research Incorporated
Part of The Investigator's Annexe program.
|Cell Type:||Hybridoma cell line|
|Antigen:||Immunoglobulin Light Chain|
|Reactivity:||Microchiropteran Bats, no reactivity against megachiroptera|
|Growth Conditions:||Complete RPMI (RPMI-1640 with 10% fetal bovine serum and supplemented with 50 uM 2-mercaptoethanol, 100U/ml penicillin, 100 ug/ml streptomycin, and 2mM glutamine)|
|Species Immunized:||BALB/c mouse|
|Cryopreservation:||Resuspend cells directly into 90% FBS, 10% DMSO. Store vials at -80C for 24-48 hrs at -70 before transfer to liquid nitrogen.|
Mouse-Anti-Bat Ig Light Chain Hybridoma
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