Anti-LIN-9 [mAb#6] Antibody

This mouse IgG1 monoclonal antibody was generated against a purified recombinant LIN-9 short-GST fusion protein and reacts with human and mouse LIN-9 (epitope: aa 360-542).

LIN-9, also known as TGS, BARA or TGS1, is a 542 amino acid protein that localizes to the nucleoplasm and is a mammalian homolog of the C. elegans Lin-9 protein. Expressed in testis and thymus, LIN-9 functions as a component of the DREAM complex (also known as the LINC complex), which is comprised of several proteins, all of which work in concert to repress cell cycle-dependent genes. LIN-9 specifically acts as a tumor suppressor that associates with Rb and inhibits DNA synthesis, possibly also controlling the expression of genes that are required for the G1/S cell cycle transition. Three isoforms of LIN-9 exist due to alternative splicing events.

From a laboratory at University of Illinois at Chicago.

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Catalog Number Product DataSheet Size AVAILABILITY Price Qty
EB6002
Anti-LIN-9 [mAb#6] Antibody
100ug 4-6 weeks
Regular Price:$278.00
On Sale:
Specifications

Product Type: Antibody
Antigen: LIN-9
Accession ID: Q5TKA1
Molecular Weight: aproximately 62 kDa (LIN-9 long), 54 kDa (LIN-9 short), and 48 kDa (LIN-9?84)
Isotype: IgG1
Clonality: Monoclonal
Clone Name: mAb#6
Reactivity: Human, mouse (others not tested)
Immunogen: full-length GST-BARA/LIN9-L fusion protein
Species Immunized: Mouse
Epitope: Amino acids 360-542
Purification Method: Protein G
Buffer: PBS, 0.05% (w/v) Sodium Azide
Tested Applications: WB, IP, IF, IHC
Concentration: 1 mg/mL
Storage: -20C
Shipped: Cold packs

Data

Characterization of LIN-9 mAbs

mAb#6 Figure

(A)Two independent mAbs were produced by immunizing BALB/c mice with a full-length GST-BARA/LIN9-L. Cell lysates obtained from 293T cells were immunoprecipitated with control IgG1 (CT), mAb#1, mAb#6 or an anti-BARA/LIN-9 pAb. Western blotting was sequentially performed with anti-BARA/LIN-9 mAb #6, mAb#1 and pAb (top, medium and bottom panels, respectively). The heavy chain of the IgG (IgG-HC) is indicated by an asterisk. The IgG-HC for mAb#1 migrates as a doublet as demonstrated by direct Western blotting in the absence of lysates (data not shown). The anti-BARA/LIN-9 pAb and mAb#6 immunoprecipitate proteins with a molecular mass of 62 and 55 kDa (long and short forms, respectively) that are detected by blotting with mAb#6 and pAb (upper and bottom panels, respectively). The mAb#1 can only recognize BARA/LIN9-L.(B) Expression of BARA in cell lines. Cell lysates (1 mg) from the indicated cell lines were immunoprecipitated with a rabbit anti-BARA/LIN-9 antibody (BARA) or normal rabbit serum (NR), followed by SDS-PAGE (8% gel), and Western blotting with the anti-BARA/LIN-9 monoclonal antibody (mAb) #6. The migration of the long and short forms is indicated by an asterisk. IgG, immunoglobulin heavy chain. The 68 and 50 kDa molecular weight markers are shown.

Adapted from: Sandoval R, et al. Exp Cell Res. 2006 Aug 1;312(13):2465-75.

Provider
From a laboratory at University of Illinois at Chicago.
References
  1. Sandoval R, Xue J, Tian X, Barrett K, Pilkinton M, Ucker DS, Raychaudhuri P, Kineman RD, Luque RM, Baida G, Zou X, Valli VE, Cook JL, Kiyokawa H, Colamonici OR. A mutant allele of BARA/LIN-9 rescues the cdk4-/- phenotype by releasing the repression on E2F-regulated genes. Exp Cell Res. 2006 Aug 1;312(13):2465-75.

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