Custom Lentivirus Production

Our custom lentivirus product service provides customers with maximal flexibility and top quality. Our services range from construct design, to lentivral vector production, concentration and titration.

Lentiviral vectors are a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, production of recombinant lentiviral vectors is laborious and challenging, especially when high titer of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) are required. 

Our standardized and optimized custom lentiviral vector production and strict quality control systems enables us to generate high titer of functional recombinant lentiviral vectors for our customers. Our titration procedures determine the quantity of functional lentiviral vectors rather than physical lentiviral particles.

Catalog Number Product Size AVAILABILITY Price Qty
FCTC02
Custom Lentivirus Production
N/A 2-3 weeks
See Price in Shopping Cart
Contact us for details.
Specifications

Product Type: Virus
Name: Custom Lentivirus
Biosafety Level: BSL-2
Vector Information: pLV-CE
Promoter: Various available
Selection Gene: Various available
Titer: See service summary table
Storage: Store at -80C. Multiple freeze/thaw cycles not recommended.
Shipped: Dry ice

Documentation

PDF Standard Plasmid Map from which Premade Lentiviruses are Generated
PDF Premade Lentivirus Transduction Protocol

 

Service Description
Titer
Volume
Turnaround Time
Price*
Custom Crude Lentiviral Vector Production
N/A
10 mL
2-3 Weeks
$350
Custom Low-Titer of Lentivirus Production
>107 TU/mL
1 mL
2-3 Weeks
$1190
Custom Medium-Titer of Lentivirus Production
>108 TU/mL
200 µL
2-3 Weeks
$1590
Custom High-Titer of Lentivirus Production
>109 TU/mL
200 µL
2-3 Weeks
$2190
Custom Ultra-High-Titer of Lentivirus Production
>1010 TU/mL
20 µL
2-3 Weeks
$2490
Custom-Titer and Scale of Lentivirus Production
Custom
Custom
2-3 Weeks
*NOTE: Price does not include construct generation.
Comments

How Our Custom Lentivirus Production Works

If your lentiviral construct is ready, please provide us:

  • The construct map or sequence.
  • 1-5 µg of your lentiviral construct plasmid DNA or bacterial culture.

We will prepare Endotoxin-free plasmid and transfect our packaging cell line for your lentivirus production. Harvest lentivirus particle followed by concentration and titration.

If you need us to generate lentiviral vector for your target gene, please:

  • Choose lentiviral vector format from our vector collection and specify the gene of interest.
  • Please send us 1-5 µg plasmid or bacterial culture for your gene if it is available, otherwise send us cDNA sequence.

We will subclone the gene of interest or shRNA into chosen lentiviral vector and confirm by sequencing, prepare high quality plasmid DNA and transfect packaging cell line, harvest lentivirus particle followed by concentration and titration.

Have Questions? Please contact us with your specific needs or any questions.

References

If you publish research with this product, please let us know so we can cite your paper.

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