The 1E12.D.1 monoclonal antibody recognizes mouse, rat and human mu-delta opioid receptor heteromers and is useful for studying G protein-coupled receptor (GPCR) signaling. This antibody was generated using a subtractive immunization strategy where mice were first tolerized to HEK membranes and then immunized with HEK membranes that co-expressed mouse mu and delta opioid receptors.
Opioid receptors are members of the G protein-coupled receptor (GPCR) superfamily characterized by the presence of seven transmembrane regions. The µ and d types of opioid receptors form heteromers that exhibit pharmacological and functional properties distinct from those of homomeric receptors.
From the laboratory of Lakshmi A. Devi, PhD, Icahn School of Medicine at Mount Sinai.
|Accession ID:||P35372 (mu), P41143 (delta)|
|Antigen:||Mu-Delta Opioid Receptor Heteromer|
|Reactivity:||Human, Mouse, Rat|
|Immunogen:||HEK membranes co-expressing mouse mu and delta opioid receptors|
|Purification Method:||Protein A|
|Buffer:||100 mM Tris-glycine, pH 7|
|Tested Applications:||ELISA (1:100-1:500), IP (1:10), IF (1:100-1:300), IHC (1:100-1:300)|
|Storage:||-20C. Once thawed, store at 4C (avoid repeat freeze thaw cycles).|
Use of Anti-Mu-Delta Opioid Receptor Heteromer (1E12.D.1) Antibody
(A) ELISA with 1E12.D.1 mAb and cortical membranes from wild-type and mu, delta or mu-delta knock-out mice. (B) Immunoprecipitation of HA mu-Flag delta opioid receptor heteromers expressed in CHO cells using the 1E12.D.1 mAb. (C) IIF analysis of cell bodies from primary DRG neurons of adult rats (Top Panel) and of dendrites from E18 rats (Bottom Panel) treated without or with 100 nM morphine for 48 h using the 1E12.D.1 mAb (red) and DAPI (blue). (D) IHC analysis of thaw-mounted 16 um thick sections from the medial nucleus of the trapezoid body of wild-type and mu or delta knock-out mice using the 1E12.D.1 mAb (green) and GOLGA5 Ab (red).
Adapted from: Gupta A, Mulder J, Gomes I, Rozenfeld R, Bushlin I, Ong E, Lim M, Maillet E, Junek M, Cahill CM, Harkany T, Devi LA. Increased abundance of opioid receptor heteromers after chronic morphine administration. Sci Signal. 2010 Jul 20;3(131):ra54.
Important: 1E12.D.1. mAb works best with native cells/tissues co-expressing mu and delta opioid receptors. For fixation of cells/tissues use mild fixatives followed by extensive wash-out of the fixatives.
Gupta A, Mulder J, Gomes I, Rozenfeld R, Bushlin I, Ong E, Lim M, Maillet E, Junek M, Cahill CM, Harkany T, Devi LA. Increased abundance of opioid receptor heteromers after chronic morphine administration. Sci Signal. 2010 Jul 20;3(131):ra54.
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