The NOY1 cell line is a novel ovarian yolk sac tumor (YST) model useful for malignant ovarian germ cell tumor (MOGCT) research. NOY1 originated from the YST of a 28-year-old female. Tumor cells were collected via right salpingo oophorectomy and passaged 50 times in order to establish the NOY1 cell line.
Yolk sac (endodermal sinus) tumors are highly malignant ovarian germ cell tumors with characteristic expression of alpha-fetoprotein (AFP, also known as alpha-1-fetoprotein and alpha-fetoglobulin), a biomarker for germ cell tumors and other hepatocellular cancers. The NOY1 cell line has been shown to form tumors in vivo; nude mice injected with NOY1 cells developed peritoneal disseminations, which stained positive for AFP via immunohistochemistry.
From a laboratory at Nagoya University.
|Product Type:||Cell Line|
|Cell Type:||Ovarian Yolk Sac Tumor|
|Source:||Human right ovary/yolk sac|
|Growth Conditions:||RPMI-1640 medium, 5% Fetal Bovine Serum, 100U/mL penicillin G, 100 ug/mL streptomycin|
|Subculturing:||A subcultivation ratio of 1:2 to 1:6 is recommended|
|Cryopreservation:||Complete growth medium supplemented with 5% (v/v) DMSO|
AFP Expression in NOY1 Human Ovarian Yolk Sac Tumor Cells
(A) AFP levels quantified by ELISA in the culture medium of NOY1 cells after 3 days. (B) Immunohistochemical analysis of AFP expression in the tumor cells of mice (magnification, x200 and x400). Scale bar = 20 um.
Adapted from: "Establishment and Characterization of an Ovarian Yolk Sac Tumor Cell Line Reveals Possible Involvement of Nkx2.5 in Tumor Development", K. Shibata, H. Kajiyama, et al., Oncology 2008; 74: 104 - 111.
A subcultivation ratio of 1:2 to 1:6 is recommended. Medium should be renewed 2-3 times per week.
If you publish research with this product, please let us know so we can cite your paper.