This IgG1a mouse antibody was generated against the N-terminus of human Nuclear Transport Factor 2 (NTF2) (amino acids 1-14) and recognizes human and xenopus NTF2.
Nucleocytoplasmic transport plays an important role in the regulation of diverse cellular processes, including transcription and translation, growthfactor-mediated signaling, stress responses, and cell cycle control. Nucleartransport factor 2 (NTF2) is a soluble transport protein originally identified by its ability to stimulate nuclear localization signal (NLS)-dependent proteinimport in digitonin-permeabilized cells. NTF2 has been shown to bind nuclearpore complex proteins and the GDP form of Ran in vitro.
From the laboratory of Bryce M. Paschal, PhD, University of Virginia.
Part of The Investigator's Annexe program.
|Antigen:||Nuclear Transport Factor 2 (NTF2) (5E8)|
|Clone Name:||5 E8|
|Reactivity:||Recognizes human and xenopus homologs of NTF2|
|Immunogen:||Generated against human NTF2 (amino acids 1-14).|
|Species Immunized:||balb/c mouse|
|Purification Method:||Protein G affinity purified|
|Tested Applications:||WB, IF|
Assignment of the epitope of anti- NTF2 5E8.
Western blot of recombinant untagged human NTF2 (15 ng), HeLa cytosol, and Xenopus lysate probed with 5E8 (1:1000 of ascites fluid). The synthetic N-terminal peptide of human NTF2 used to generate 5E8 is shown above the corresponding Nterminal 14 amino acids from Xenopus NTF2. Residues conserved between human and Xenopus NTF2 are in bold. The bracket indicates residues that likely encompass the 5E8 epitope. (Adapted from: Steggerda SM, Black BE, and Paschal BM. Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran. Mol Biol Cell. 11(2):703-19 (2000).).
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