This IgG2a mouse monoclonal antibody was generated against human Nuclear Transport Factor 2 (NTF2) and recognizes human and xenopus homologs.
Nucleocytoplasmic transport plays an important role in the regulation of diverse cellular processes, including transcription and translation, growth factor-mediated signaling, stress responses, and cell cycle control. Nuclear transport factor 2 (NTF2) is a soluble transport protein originally identified by its ability to stimulate nuclear localization signal (NLS)-dependent protein import in digitonin-permeabilized cells. NTF2 has been shown to bind nuclear pore complex proteins and the GDP form of Ran in vitro.
From the laboratory of Bryce M. Paschal, PhD, University of Virginia.
Part of The Investigator's Annexe program.
|Antigen:||Nuclear Transport Factor 2 (NTF2) (5A3)|
|Reactivity:||Recognizes human and xenopus homologs|
|Species Immunized:||balb/c mouse|
|Purification Method:||Protein G affinity purified|
|Buffer:||PBS, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, IF|
Characterization of anti-NTF2 (5A3) mAb.
Anti-NTF2 mAbs are monospecific for NTF2. Recombinant human untagged (15 ng) or myc-tagged (85 ng) NTF2 protein and HeLa cytosol were separated on 15% SDS-PAGE gels, transferred to nitrocellulose, and probed with anti-NTF2 mAb. 5A3 was used at 5 mg/ml purified antibody. (Adapted from: Steggerda SM, Black BE, and Paschal BM. Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran. Mol Biol Cell. 11(2):703-19 (2000). )
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