Anti-NTF2 [5A3] Antibody

This IgG2a mouse monoclonal antibody was generated against human Nuclear Transport Factor 2 (NTF2) and recognizes human and xenopus homologs.

Nucleocytoplasmic transport plays an important role in the regulation of diverse cellular processes, including transcription and translation, growth factor-mediated signaling, stress responses, and cell cycle control. Nuclear transport factor 2 (NTF2) is a soluble transport protein originally identified by its ability to stimulate nuclear localization signal (NLS)-dependent protein import in digitonin-permeabilized cells. NTF2 has been shown to bind nuclear pore complex proteins and the GDP form of Ran in vitro.

From the laboratory of Bryce M. Paschal, PhD, University of Virginia.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EG6001
Anti-NTF2 [5A3] Antibody
100ug 2-3 weeks
Regular Price:$344.00
Specifications

Product Type: Antibody
Antigen: Nuclear Transport Factor 2 (NTF2) (5A3)
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: 5A3
Reactivity: Recognizes human and xenopus homologs
Species Immunized: balb/c mouse
Purification Method: Protein G affinity purified
Tested Applications: WB, IF
Concentration: 1mg/mL
Storage: -20C
Shipped: Cold packs

Documentation

Characterization of anti-NTF2 (5A3) mAb.

Characterization of anti-NTF2 (5A3) mAb.

Anti-NTF2 mAbs are monospecific for NTF2. Recombinant human untagged (15 ng) or myc-tagged (85 ng) NTF2 protein and HeLa cytosol were separated on 15% SDS-PAGE gels, transferred to nitrocellulose, and probed with anti-NTF2 mAb. 5A3 was used at 5 mg/ml purified antibody. (Adapted from: Steggerda SM, Black BE, and Paschal BM. Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran. Mol Biol Cell. 11(2):703-19 (2000). )

Provider
From the laboratory of Bryce M. Paschal, PhD, University of Virginia.
References
  1. Steggerda SM, Black BE, and Paschal BM. Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran. Mol Biol Cell. 11(2):703-19 (2000).

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