This mouse IgG1 antibody was generated against amino acids 119-416 of human NORE1A and the epitope has been mapped to amino acids 119-190, a unique sequence in the NORE1A splice isoform.
NORE1 (novel Ras effector 1) is a growth and tumor suppressor that is inactivated in a variety of cancers. Full-length NORE1 is 47 kDa and contains a proline-rich region at its N terminus, a putative diacylglycerol/phorbol ester-binding domain, and a C-terminal canonical Ras binding domain. The human NORE1 gene encodes several isoforms, of which NORE1A and NORE1B are the most prominent.
From the laboratory of Andrei Khokhlatchevz, PhD, University of Virginia.
Part of The Investigator's Annexe program.
|Name:||Anti-NORE1A, Human (10F10-C6-C6) Monoclonal Antibody|
|Specificity:||Recognizes human NORE1A|
|Immunogen:||Generated against amino acids 119-416 of human NORE1A|
|Purity:||Protein G affinity purified|
|Tested Applications:||WB, IF|
|Storage:||Store at 4C|
Expression of human NORE1A in human cells. Cells were lysed in radioimmune precipitation (RIPA) buffer, and an equal amount of protein was separated by polyacrylamide gel and probed on Western blot with anti-NORE1A monoclonal antibody 10F10 (upper panel) or for alpha-tubulin (lower panel).
Adapted from: Moshnikova A, Frye J, Shay JW, Minna JD, Khokhlatchev AV. The growth and tumor suppressor NORE1A is a cytoskeletal protein that suppresses growth by inhibition of the ERK pathway. The Journal of Biological Chemistry. 281(12): 8143-8152 (2006).
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