This mouse IgG1 antibody was generated against human activated tonsillar B cells and is specific for human CD40.
B cells require a series of coordinated signals in order to undergo maturation including MHC antigen presentation, cytokine stimulation (IL-2/4/5) and costimulation. CD40 is a costimulatory protein found on antigen presenting cells (such as B Cells) and is required for their activation. The binding of CD154 (CD40L) on T helper cells to the CD40 receptor stimulates antigen presenting cells to undergo maturity and develop downstream effector functions.
From the laboratory of Shu-Man Fu, MD, University of Virginia.
Part of The Investigator's Annexe program.
|Name:||Anti-CD40, Human S19 (626)|
|Immunogen:||Generated against human activated tonsillar B cells|
|Purification Method:||Protein G affinity purified|
|Buffer:||PBS, 0.05% (w/v) Sodium Azide|
|Tested Applications:||FC, IHC, WB|
|Storage:||Store at -20C|
Immunopreclpltatlons wlth detergent extracts of 125I-labeled tonsillar B cells. Lysates were treated with complexes of goat anti-mouse with anti-CD40-626 mAb and goat anti-mouse Ig. The precipitates were run on a 10% SDS-PAGE under reducing conditions. Control Ig mAb HDP-1 (lane a) and anti-CD40-626 (lane b).
Adapted from: Gruber MF, Bjorndahl JM, Nakamura S, Fu SM. Anti-CD45 inhibition of human B cell proliferation depends on the nature of activation signals and the state of B cell activation. J Immunol, 142(12):4144-4152, (1989).
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