This mouse IgG1 antibody was generated against the PH domain (amino acids 1-175) of human BTK.
BTK (Bruton's tyrosine kinase), also known as BPK or ATK, is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. BTK plays a crucial role in B cell activation and development. Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner. Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA).
From the laboratory of Shu-Man Fu, MD, University of Virginia.
Part of The Investigator's Annexe program.
|Name:||Anti-BTK, Human (10D11)|
|Immunogen:||Generated against the PH domain (amino acids 1-175 of human BTK)|
|Purification Method:||Protein G affinity purified|
|Buffer:||PBS, 0.05% (w/v) Sodium Azide|
|Tested Applications:||WB, IP|
|Storage:||Store at -20C|
Expression of Btk in normal and patient PBMC. The protein levels of Btk in total PBMC and fractionated cell populations were examined by western blotting. PBMC and purified monocytes (M), T cells (T), and B cells (B) from the same buffy coat were used. The numbers above the lanes indicate the number of cells x 106 used in each assay. Arrow indicates the position of Btk.
Adapted from: Bykowsky MJ, Haire RN, Ohta Y, Tang H, Sung SS, Veksler EG, Greene JM, Fu SM, Litman GW, Sullivan KE. Discordant phenotype in siblings with X-linked agammaglobulinemia. Am J Hum Genet, 58(3):477-483, (1996).
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