E1A-NR.3 was established by immortalization of postnatal day 6 rat neuroretinal tissue using the psi2 replication incompetent retroviral vector. As a result, these cells are already resistant to geneticin/G418 and would require an alternative selection marker for transfection studies. These cells were designed not to form tumors in animals.
Read Dr. Seigel's related blog post, On the Road to Retinal Cells »
From the laboratory of Gail M. Seigel, PhD, University of Rochester.
Part of The Investigator's Annexe program.
|Product Type:||Cell Line|
|Cell Type:||12S E1A-immmortalized rat retinal cells|
|Morphology:||Adherent with glial and neuronal morphologies|
|Source:||Postnatal day 6 rat neural retina|
|Subculturing:||Split 1:2 when 80% confluent|
|Growth Conditions:||DMEM with 10% calf serum|
|Cryopreservation:||90% culture medium with 10% DMSO|
E1A-immortalized cell characterization
E1A-immortalized cell utilization
If you publish research with this product, please let us know so we can cite your paper.