The R28 cells were developed from E1A-NR.3 parental cell line through three rounds of limiting dilution and were therefore derived from a single cell. Despite their clonal origin, these cells display both glial and neuronal cell markers indicative of a retinal precursor cell.
The parental line E1A-NR.3 was established by immortalization of postnatal day 6 rat neuroretinal tissue using the psi2 replication incompetent retroviral vector. As a result, these cells are already resistant to geneticin/G418 and would require an alternative selection marker for transfection studies. These cells were designed not to form tumors in animals.
From the laboratory of Gail M. Seigel, PhD, University of Rochester.You can now pre-order Dr. Seigel's book Academania: My Life in the Trenches of Biomedical Research.
New publication: R28 Retinal Precursor Cells: The First 20 Years
New publication: A Microarray Dataset of Genes Expressed by the R28 Retinal Cell Line
Read Dr. Seigel's related blog post, On the Road to Retinal Cells.
|Product Type:||Cell Line|
|Cell Type:||12S E1A-immmortalized rat retinal cells|
|Source:||Postnatal day 6 rat neural retina|
|Organism:||Rat; NOTE: The R28 cells have undergone verification by IDEXX-RADIL to be of rat origin without contamination by other mammalian cell lines. A baseline genetic profile of these cells is available upon request.|
|Morphology:||Adherent with glial and neuronal morphologies|
|Subculturing:||Split 1:2 when 80% confluent (See also: R28 Cell Care Instructions)|
|Growth Conditions:||DMEM+; See recipe in the recommended SOP:|
|Cryopreservation:||90% culture medium with 10% DMSO|
R28-immortalized cell characterization
R28-immortalized cell utilization
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