This antibody recognizes human and mouse PAK1 and PAK2.
P21 protein-activated kinase (PAK) proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activated kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac, and have been implicated in a wide range of biological activities. PAK activity has been linked to tumor invasiveness and motility of a variety of human cancer cell lines, and, more specifically, PAK1 appears to function in regulating the actin cytoskeleton at the leading edge of the cells, where it regulates changes required for the motility in mammalian cells.
From the laboratory of Celine DerMardirossian, PhD, The Scripps Research Institute
Part of The Investigator's Annexe program.
|Name:||Anti-PAK1 (2124) Polyclonal Antibody|
|Accession ID:||NG_029900, Q13153|
|Molecular Weight:||65kDa (PAK1), 62kDA (PAK2)|
|Immunogen:||Antibody against PAK1 sequence (amino acids 174-306)|
|Tested Applications:||WB (1:2000)|
|Storage:||Store at 4C or -20C|
No cross-reactivity with PAK3
Endogenous Pak1 and Pak2 expression levels in cells. (Left) Lysates from PtK1 cells were immunoblotted for endogenous Pak1 and Pak2 using either a rabbit polyclonal antisera (anti-Pak1 2124) that recognizes both isoforms, an anti-Pak1 antibody (rabbit polyclonal), or an anti-Pak2 antibody (rabbit monoclonal). (Right) Lysates from PtK1 (ratkangaroo kidney epithelial cells), U2OS (human osteosarcoma), B16F10 (mouse melanoma), B16F1 (mouse melanoma), and mouse embryonic fibroblast (MEF) cells were immunoblotted for endogenous Pak1 and Pak2 (anti-Pak1 2124) and actin (mouse monoclonal C4). Molecular mass is indicated in kilodaltons.
Adapted from: Delorme-Walker VD, Peterson JR, Chernoff J, Waterman CM, Danuser G, DerMardirossian C, Bokoch GM. Pak1 regulates focal adhesion strength, myosin IIA distribution, and actin dynamics to optimize cell migration. J Cell Biol. 193(7):1289-303, (2011).
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