Anti-PAK1 [2124] Antibody

This antibody recognizes human and mouse PAK1 and PAK2.

P21 protein-activated kinase (PAK) proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activated kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac, and have been implicated in a wide range of biological activities. PAK activity has been linked to tumor invasiveness and motility of a variety of human cancer cell lines, and, more specifically, PAK1 appears to function in regulating the actin cytoskeleton at the leading edge of the cells, where it regulates changes required for the motility in mammalian cells.

From the laboratory of Celine DerMardirossian, PhD, The Scripps Research Institute

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EST101
Anti-PAK1 [2124] Antibody
100ug In stock
Regular Price:$311.00
Specifications

Product Type: Antibody
Name: Anti-PAK1 (2124) Polyclonal Antibody
Accession ID: NG_029900, Q13153
Molecular Weight: 65kDa (PAK1), 62kDA (PAK2)
Clonality: Polyclonal
Clone Name: 2124
Reactivity: Human, Mouse
Immunogen: Antibody against PAK1 sequence (amino acids 174-306)
Species Immunized: Rabbit
Purification Method: Serum
Tested Applications: WB (1:2000)
Concentration: 1 mg/mL
Storage: Store at 4C or -20C
Shipped: Cold packs

Provider
From the laboratory of Celine DerMardirossian, PhD, The Scripps Research Institute
Comments

No cross-reactivity with PAK3

Pak1

Endogenous Pak1 and Pak2 expression levels in cells. (Left) Lysates from PtK1 cells were immunoblotted for endogenous Pak1 and Pak2 using either a rabbit polyclonal antisera (anti-Pak1 2124) that recognizes both isoforms, an anti-Pak1 antibody (rabbit polyclonal), or an anti-Pak2 antibody (rabbit monoclonal). (Right) Lysates from PtK1 (rat–kangaroo kidney epithelial cells), U2OS (human osteosarcoma), B16F10 (mouse melanoma), B16F1 (mouse melanoma), and mouse embryonic fibroblast (MEF) cells were immunoblotted for endogenous Pak1 and Pak2 (anti-Pak1 2124) and actin (mouse monoclonal C4). Molecular mass is indicated in kilodaltons.

Adapted from: Delorme-Walker VD, Peterson JR, Chernoff J, Waterman CM, Danuser G, DerMardirossian C, Bokoch GM. Pak1 regulates focal adhesion strength, myosin IIA distribution, and actin dynamics to optimize cell migration. J Cell Biol. 193(7):1289-303, (2011).

References
  1. Delorme-Walker VD, Peterson JR, Chernoff J, Waterman CM, Danuser G, DerMardirossian C, Bokoch GM. Pak1 regulates focal adhesion strength, myosin IIA distribution, and actin dynamics to optimize cell migration. J Cell Biol. 193(7):1289-303, (2011).
  2. Knaus UG, Morris S, Dong HJ, Chernoff J, Bokoch GM. Regulation of human leukocyte p21-activated kinases through G protein--coupled receptors. Science. 269(5221): 221-223, (1995).
  3. "Wang J, MacEwan SR, Chilkoti A. Quantitative Mapping of the Spatial Distribution of Nanoparticles in Endo-Lysosomes by Local pH. Nano Lett. 2017 Jan 3. doi: 10.1021/acs.nanolett.6b05041. [Epub ahead of print] PubMed PMID: 28033711." View Article

If you publish research with this product, please let us know so we can cite your paper.

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