This plasmid expresses the full length E. coli Histidyl-tRNA synthetase.
Histidyl-tRNA synthetases attach amino acids to the 3' ends of their cognate transfer RNAs in a two-step reaction catalyzed by ATP. The accuracy of this reaction is crucial for the overall fidelity of protein synthesis.
From the laboratory of Christopher S. Francklyn, PhD, University of Vermont.
Part of The Investigator's Annexe program.
|Name:||Histidine tRNA Synthetase|
|Grow in E. coli at 37 C:||Yes|
|Cloning Site 5':||NcoI|
|Cloning Site 3':||BamHI|
|5' Sequencing Primer:||1272 bp|
|3' Sequencing Primer:||pTRC99, 4167 bp|
|Shipped:||Ambient temperature (Liquid plasmid DNA in water for domestic orders, spotted on filter paper for international orders)|
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