This mouse IgG2a monoclonal antibody was generated against recombinant human KLF6 splice variant 1 (SV1), and is specific for human KLF6 SV1.
Krueppel-like factor 6 (KLF6), also known as GBF, ZF9, BCD1, CPBP, PAC1, ST12, and COPEB, is a protein that in humans is encoded by the KLF6 gene. It is a tumor suppressor gene and encodes a nuclear protein that has three zinc fingers at the end of its C-terminal domain, a serine/threonine-rich central region, and an acidic domain lying within the N-terminal region. The zinc fingers of this protein are responsible for the specific DNA binding with the guanine-rich core promoter elements. The central region is involved in activation or posttranslational regulatory pathways, and the acidic N-terminal domain is proposed to play an important role in the process of transcriptional activation.
KLF6 is capable of activating transcription approximately 4-fold either on homologous or heterologous promoters. Although wild-type KLF6 acts as a tumor suppressor, KLF6 splice variant 1 (sv1) has the opposite effect on cell proliferation, colony formation and invasion. Inhibition of KLF6 sv1 with short interfering RNA reduces tumor cell growth by 50% and decreases the number of growth and angiogenesis-related genes.
From the laboratory of Goutham Narla, MD, PhD, Icahn School of Medicine at Mount Sinai.
Part of The Investigator's Annexe program.
|Name:||Anti KLF6 monoclonal antibody (9A2)|
|Specificity:||This antibody recognizes Human KLF6 splice variant 1 (sv1)|
|Immunogen:||Human KLF6 splice variant 1 recombinant protein|
|Purity:||Protein G purified|
|Tested Applications:||IHC, WB, ELISA|
|Storage:||Store at 4C|
Increased KLF6-SV1 expression in metastatic PCa. Western blot analysis using a KLF6-SV1specific monoclonal antibody. Transfected KLF6-SV1 and transfected wild-type KLF6 controls were run on the same gel but were noncontiguous. Tubulin was used as the loading control for all lanes.
Adapted from: Narla, G., et al., KLF6-SV1 overexpression accelerates human and mouse prostate cancer progression and metastasis. J. Clin. Invest. 118:27112721 (2008).
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