Anti-KLF6 [2A2] Antibody

This mouse IgG2a monoclonal antibody was generated against a human KLF6 synthetic peptide, and is specific for human KLF6.

Krueppel-like factor 6 (KLF6), also known as GBF, ZF9, BCD1, CPBP, PAC1, ST12, and COPEB, is a protein that in humans is encoded by the KLF6 gene. It is a tumor suppressor gene and encodes a nuclear protein that has three zinc fingers at the end of its C-terminal domain, a serine/threonine-rich central region, and an acidic domain lying within the N-terminal region. The zinc fingers of this protein are responsible for the specific DNA binding with the guanine-rich core promoter elements. The central region is involved in activation or posttranslational regulatory pathways, and the acidic N-terminal domain is proposed to play an important role in the process of transcriptional activation.

KLF6 is capable of activating transcription approximately 4-fold either on homologous or heterologous promoters. Although wild-type KLF6 acts as a tumor suppressor, KLF6 splice variant 1 (sv1) has the opposite effect on cell proliferation, colony formation and invasion. Inhibition of KLF6 sv1 with short interfering RNA reduces tumor cell growth by 50% and decreases the number of growth and angiogenesis-related genes.

From the laboratory of Goutham Narla, MD, PhD, Icahn School of Medicine at Mount Sinai.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EMS001
Anti-KLF6 [2A2] Antibody
100ug 2-3 weeks
Regular Price:$338.00
Specifications

Product Type: Antibody
Name: Anti KLF6 monoclonal antibody (2A2)
Host: Mouse
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: 2A2
Specificity: This antibody recognizes Human KLF6
Immunogen: Human KLF6 synthetic peptide
Format: Liquid
Purity: Protein G purified
Tested Applications: WB, ELISA
Concentration: 1 mg/mL
Amount: 100uL
Storage: Store at 4C
Shipped: Cold packs

Data

WB Analysis of Mouse Livers for KLF6. Mouse livers were homogenized. Lysates were separated by polyacrylamide gel electrophoresis. Gels were transferred to a nitrocellulose membrane that was blotted for KLF6 (1:250; Rabbit 1:2500; Anti KLF6), p21 and GAPDH. KLF6 Het mice had significantly reduced expression of KLF6 and p21 protein compared with WT littermates.

Adapted from: Narla et al. In vivo regulation of p21 by the Kruppel-like factor 6 tumor-suppressor gene in mouse liver and human hepatocellular carcinoma.Oncogene, 26, 4428, 2007

Provider
From the laboratory of Goutham Narla, MD, PhD, Icahn School of Medicine at Mount Sinai.
References
  1. Narla et al. In vivo regulation of p21 by the Kruppel-like factor 6 tumor-suppressor genein mouse liver and human hepatocellular carcinoma.Oncogene, 26, 4428, 2007.
  2. Narla et al, KLF6, a Candidate TumorSuppressor Gene Mutated in Prostate Cancer.Science, 294, 2563, 2001.

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