This antibody was raised in mice against rat eIF4G1 (residues 96-600) that was expressed in and purified from Sf9 cells.
The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the Eukaryotic initiation factor 4G (eIF4G) translation factor family bind to the 5' mRNA cap and unwind the mRNA secondary structure.
eIF4G (eukaryotic translation initiation factor 4 gamma) is a protein involved in bringing mRNA to the ribosome for translation, in general based on mRNA's distinctive guanosine cap.The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure. eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex. Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation.
From the laboratory of Scot R. Kimball, PhD, Penn State College of Medicine
Part of The Investigator's Annexe program.
|Name:||Anti-eIF4G1 monoclonal antibody (4GR11)|
|Specificity:||This antibody recognizes human, mouse, rat, and pig eIF4G1|
|Immunogen:||Fragment corresponding to approx. amino acids 96-600 of the rat protein|
|Purification Method:||HiTrap Protein G HR chromatography|
|Tested Applications:||Western blotting, ELISA, IP, and IF.|
|Storage:||Store at 4C|
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