This antibody was raised in mice against human eukaryotic initiation factor 4E (eIF4E) that was expressed in and purified from Sf9 cells.
The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the Eukaryotic initiation factor 4 (eIF4) translation factor family bind to the 5' mRNA cap and unwind the mRNA secondary structure. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methyl-guanosine cap structure. This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export.
eIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. eIF4E's function is to bind an mRNA cap and ultimately bring it to the ribosome. eIF4E is part of the eIF4F pre-initiation complex, which is made up of eIF4E, and eIF4G. Almost all cellular mRNA's require eIF4E in order to be translated into protein.
From the laboratory of Scot R. Kimball, PhD, Penn State College of Medicine
Part of The Investigator's Annexe program.
|Name:||Anti-eIF4E monoclonal antibody (4GRH08)|
|Specificity:||This antibody recognizes human, mouse, rat, and pig eIF4E|
|Purification Method:||HiTrap Protein G HR chromatography|
|Tested Applications:||Western blotting, ELISA, IP, and IF.|
|Storage:||Store at 4C|
Western Blot: The blot shown above was incubated with anti-eIF4E antibody at a 1:3,000 dilution followed by secondary antibody (Bethyl Laboratory) at a 1:10,000 dilution. The blot was developed with ECL reagent. Equal amounts of protein from HEK293 (human), C2C12 (mouse), and Rat 2 (rat) were analyzed.
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