Immortalized Bovine Satellite Cell Line (iBSC1)
iBSC1 is an immortalized bovine satellite cell line, isolated from the semitendinosus muscle of a female Simmental calf (bos taurus). The cells were generated through Sleeping Beauty transposon-mediated insertion of bovine TERT and CDK4 under constitutive promotion by CMV and RPBSA, respectively.
Highlights:
- Contains a puromycin resistance gene
- Overexpresses bovine TERT and CDK4
- Useful for cellular agriculture and cultured meat applications
- Offers robust proliferation while maintaining its myogenic phenotype
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
iBSC1 is an immortalized bovine satellite cell line, isolated from the semitendinosus muscle of a female Simmental calf (bos taurus). The cells were generated through Sleeping Beauty transposon-mediated insertion of bovine TERT and CDK4 under constitutive promotion by CMV and RPBSA, respectively.
Highlights:
- Contains a puromycin resistance gene
- Overexpresses bovine TERT and CDK4
- Useful for cellular agriculture and cultured meat applications
- Offers robust proliferation while maintaining its myogenic phenotype
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
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Specifications
| Product Type: | Cell Line |
| Name: | iBSC1 |
| Cell Type: | Muscle satellite cells |
| Morphology: | Myogenic |
| Organism: | Bos taurus |
| Source: | Semitendinosus muscle |
| Biosafety Level: | BSL-1 |
| Growth Conditions: | DMEM + 20% FBS + 1 ng/mL FGF-2 + 2.5 ug/mL puromycin |
| Subculturing: | Doubling time: ~15 hours. Subculture at ~70% confluency. Plate ~2000/cm^2. |
| Cryopreservation: | Growth media with 10% DMSO |
| Comments: |
Contains a puromycin resistance gene. To differentiate, culture cells to confluency and change to differentiation medium (e.g., DMEM + 2% FBS). |
| Storage: | LN2 |
| Shipped: | Dry ice |
Provider
From the laboratory of David Kaplan, PhD, Tufts University Center for Cellular Agriculture (TUCCA).
References
- Stout AJ, Arnett MJ, Chai K, et al. Immortalized Bovine Satellite Cells for Cultured Meat Applications. ACS Synth Biol. 2023;12(5):1567-1573. doi:10.1021/acssynbio.3c00216
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