Anti-H3K4me3 Antibody
This H3K4me3 rabbit polyclonal antibody was raised against a modified branched peptide and recognizes histone H3 tri-methylated on lysine 4 (H3K4me3).
Highlights:
- Detects a single protein of approximately 14 kDa by western blot using acid extracted histones
- Suitable for chromatin immunoprecipitation (ChIP) assays
H3K4me3 indicates the addition of a trimethyl group to the 4th lysine residue on the histone H3 protein, and is often associated with transcription of nearby genes. This trimethylation regulates gene expression through chromatin remodeling by the NURF complex. H3K4me3 has an important role in stem cell potency and early embryogenesis, DNA repair, and is commonly used as a histone mark in epigenetic studies.
This H3K4me3 rabbit polyclonal antibody was raised against a modified branched peptide and recognizes histone H3 tri-methylated on lysine 4 (H3K4me3).
Highlights:
- Detects a single protein of approximately 14 kDa by western blot using acid extracted histones
- Suitable for chromatin immunoprecipitation (ChIP) assays
H3K4me3 indicates the addition of a trimethyl group to the 4th lysine residue on the histone H3 protein, and is often associated with transcription of nearby genes. This trimethylation regulates gene expression through chromatin remodeling by the NURF complex. H3K4me3 has an important role in stem cell potency and early embryogenesis, DNA repair, and is commonly used as a histone mark in epigenetic studies.
Specifications
| Product Type: | Antibody |
| Antigen: | H3K4me3 |
| Clonality: | Polyclonal |
| Clone Name: | H3K4me3 |
| Immunogen: | Modified branched peptide |
| Species Immunized: | Rabbit |
| Buffer: | Whole serum |
| Tested Applications: | ChIP assay (ideal for ChIP-seq or ChIP-qPCR) |
| Comments: | ChIP Recommendations: Use 2.5-5.0 ul of crude sera with 40-60 ul of Protein A beads per 100 ug of sheared chromatin lysate. We recommend titrating the antibody, beads, and lysate to determine ideal concentrations. We also recommend pre-binding the antibody to beads. |
| Storage: | -80C |
| Shipped: | Cold Packs (Domestic, Overnight); Dry Ice (International) |
Provider
From the laboratory at Apheon Bioscience.
Comments
The antibody detects a single protein of approximately 14 kDa by western blot using acid extracted histones. The antibody has been tested in chromatin immunoprecipitation (ChIP) experiments against a strain lacking all H3K4 methyltransferase activity and determined to be specific.
References
If you publish research with this product, please let us know so we can cite your paper.
