Anti-Beta Tubulin Class VI [AA16] Antibody

AA16 recognizes the human Class VI Beta Tubulin isotype. The antibody does not recognize any other Beta Tubulin isotype, and it does not react with the rodent Class VI.

Class VI Beta Tubulin (Tubb1) is present exclusively in megakaryocytes and their derivatives, platelets. Transgenic mice lacking Class VI have non-discoid platelets with defective marginal bands, and suffer from thrombocytopenia and excessive bleeding.

From the laboratories of Anthony Frankfurter, PhD and Anthony Spano, PhD, University of Virginia.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EG0031
Anti-Beta Tubulin Class VI [AA16] Antibody
100ug In stock
Regular Price:$306.00
Specifications

Product Type: Antibody
Name: Anti-Class VI Beta Tubulin (Tubb1); clone AA16
Host: balb/c mouse
Isotype: IgG2a
Clonality: Monoclonal
Clone Name: AA16
Specificity: Human Class VI Beta Tubulin (does not react with any other Beta Tubulin isotype)
Immunogen: KLH-DEEVTEEAEMEPEDKGH (KLH peptide conjugate)
Format: Liquid
Purity: Protein G affinity purified
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications: WB (1:1000 - 5000), ELISA, IF (ICC), IHC
Concentration: 1 mg/mL
Amount: 100uL
Storage: Store at 4C
Shipped: Cold packs

Data

Pattern of reactivity of Anti-?-Tubulin antibodies against MBP-C-terminal ?-Tubulin fusion proteins. The following MBP-C-terminal ?-Tubulin fusion proteins were spotted: Class I ?-Tubulin, Class II ?-Tubulin, Class III ?-Tubulin, Class IVa ?-Tubulin, Class IVb ?-Tubulin, Class V ?-Tubulin, Class VI ?-Tubulin. Antibody for Class III ?-Tubulin, Class VI ?-Tubulin (AA16), or none was applied to spots.

Figure courtesy of: A. Frankfurter and A. Spano (unpublished data).

Provider
From the laboratories of Anthony Frankfurter, PhD and Anthony Spano, PhD, University of Virginia.
References
  1. Sullivan KF. Structure and utilization of tubulin isotypes. Ann. Rev. Cell Biology. Annu Rev Cell Biol. 1988;4:687-716.
  2. Italiano JE Jr, Bergmeier W, Tiwari S, Falet H, Hartwig JH, Hoffmeister KM, André P, Wagner DD, Shivdasani RA. Mechanisms and implications of platelet discoid shape. Blood. 2003 Jun 15;101(12):4789-96.
  3. Schwer HD, Lecine P, Tiwari S, Italiano JE Jr, Hartwig JH, Shivdasani RA. A lineage-restricted and divergent beta-tubulin isoform is essential for the biogenesis, structure and function of blood platelets. Curr Biol. 2001 Apr 17;11(8):579-86.
  4. Pulecio J, Alejo-Valle O, Capellera-Garcia S, Vitaloni M, Rio P, Mejía-Ramírez E, Caserta I, Bueren JA, Flygare J, Raya A. Direct Conversion of Fibroblasts to Megakaryocyte Progenitors. Cell Rep. 2016 Oct 11;17(3):671-683.View Article

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