Scomber scombrus Myoblast Cell Line (MACK1)

MACK1 (myoblast cell) is a continuous, adherent cell line derived from mature muscle of Atlantic mackerel (Scomber scombrus).

Highlights:

  • Cell line is useful for cellular agriculture
  • Cells progressed through a likely spontaneous immortalization crisis event, after which the cell doubling time decreased to ~30-35 hours
  • PCR and Sanger Sequencing on the cells' genomic DNA confirmed cells' identity as Scomber scombrus
  • Highly pure satellite cell population confirmed through Pax7 staining and efficient muscle differentiation

MACK1 is the first commercially available fish muscle cell line and can prove a valuable tool for studying marine cell culture. As Atlantic mackerel is a commonly consumed fish, the food-relevant cell line is especially valuable in cultivated seafood applications.

From the laboratory of David Kaplan, PhD, Tufts University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ETU008-FP
Scomber scombrus Myoblast Cell Line (MACK1)
1 vial In stock
Regular Price:$658.00
On Sale:

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Specifications

Product Type: Cell Line
Name: MACK1
Cell Type: Muscle cell line
Organism: Atlantic mackerel
Source: White muscle
Biosafety Level: BSL-2
Subculturing: Split with 0.05% or 0.25% trypsin-EDTA when cells reach 70-80% confluence. Recommended seeding density: 3,000-6,000 cells/cm2.
Growth Conditions: Growth media formulation: Leibovitz's L-15 Medium with 20% fetal bovine serum (FBS), 20 mM HEPES pH7.4, 1 ng/mL FGF-2, 1% antibiotic-antimycotic, and 10 µg/mL gentamicin. Culture the cells at 27°C without CO2 in sealed flasks.
With the first thaw, plate all 1 million cells in the vial into a 25 cm2 tissue culture flask. Allow cells 1-2 days to recover before changing the medium. The high seeding density is necessary due to poor cell viability after freezing/thawing.
Cryopreservation: Growth media with 10% DMSO
Comments: Doubling time: ~30-35 hours.; For thawing cells, seed the cells in a T25 tissue culture flask. The high seeding density is necessary due to poor cell viability after freezing/thawing.
NOTE: The cells are difficult to visualize on brightfield microscopy. Phase contrast is recommended.
Storage: LN2
Shipped: Dry ice

Provider
From the laboratory of David Kaplan, PhD, Tufts University.
References
  1. Michael K Saad, John SK Yuen Jr, Connor M Joyce, Xinxin Li, Taehwan Lim, Talia L Wolfson, Justin Wu, Jason Laird, Sanjana Vissapragada, Olivia P Calkins, Adham Ali, David L Kaplan. Continuous Fish Muscle Cell Line with Capacity for Myogenic and Adipogenic-like Phenotypes. bioRxiv 2022.08.22.504874; doi: https://doi.org/10.1101/2022.08.22.504874

If you publish research with this product, please let us know so we can cite your paper.

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