GAD65 (Syn1) pFSW67 Lentivirus Plasmid

Lentivirus construct encoding human glutamic acid decarboxylase 65 kDa isoform (GAD65, GAD2) driven by Synapsin (Syn1) promoter.

Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme identified as a major autoantigen in insulin-dependent diabetes. It is expressed in the brain as well as the insulin-producing β-cells of the pancreas. Its role is to catalyze the decarboxylation of glutamate to gamma-aminobutyric acid (GABA) and carbon dioxide (CO2). There are two isoforms of GAD that are present in mammals: GAD67 and GAD65. These are encoded by two genes, GAD1 and GAD2, respectively, located on different chromosomes (chromosomes 2 and 10 in humans). GAD2 may play a role in the stiff man syndrome, as well as other areas of pathology (such as autism, Parkinson's disease, schizophrenia and bipolar disorders, cerebellar disorders and more).

From the laboratory of Soham Chanda, PhD, Colorado State University.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ECS022
GAD65 (Syn1) pFSW67 Lentivirus Plasmid
250ng In stock
Regular Price:$66.00
On Sale:
Specifications

Product Type: Plasmid
Name: GAD65 (Syn1) pFSW67 Lentivirus Plasmid
Gene/insert name: GAD65, GAD2
Organism: Human
Antibiotic Resistance: Ampicillin
Fusion Tag(s): None
Grow in E. coli at 37 C: Yes
Selectable markers: None
Cloning Site 5': XbaI
Cloning Site 3': AscI
Insert Size: 1758 bp
Vector Backbone and Size: pFSW67, 8377 bp
High or low copy: High
Promoter: Driven by Synapsin (Syn1) promoter
Storage: -20C
Shipped: Room temperature

Provider
From the laboratory of Soham Chanda, PhD, Colorado State University.
References
  1. Burlingham SR, Wong NF, Peterkin L, Lubow L, Dos Santos Passos C, Benner O, Ghebrial M, Cast TP, Xu-Friedman MA, Südhof TC, Chanda S. Induction of synapse formation by de novo neurotransmitter synthesis. Nat Commun. 2022 Jun 1;13(1):3060. doi: 10.1038/s41467-022-30756-z. PMID: 35650274; PMCID: PMC9160008.

If you publish research with this product, please let us know so we can cite your paper.

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