This monoclonal antibody is specific to rat and mouse exocyst complex sec15 subunit (EXOC6).
Exocyst complex (also known as the sec6/8 complex) is a multimeric complex that has been implicated to play a role in exocytosis. This complex is conserved from yeast to mammals and is composed of eight subunits (Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84). In the budding yeast Saccharomyces cerevisiae, mutations in these complex subunits promote cytoplasmic accumulation of secretory vesicles and affects polarized growth. In cultured Drosophila and mammlian cells, disruption of exocyst subunit function has been found to decrease neurite outgrowth and Golgi-to-plasma membrane vesicle trafficking. Furthermore, a delay in neuronal induction was observed in mouse embryos with deletion of the sec8 gene, suggesting that the exocyst complex may play a role in cell differentiation and polarized growth.
From the laboratory of Shu-Chan Hsu, PhD, Rutgers University.
|Name:||Anti-exocyst complex sec15 subunit (15S2G6 ) monoclonal antibody|
|Isotype:||IgG subtype not characterized|
|Specificity:||This antibody recognizes exocyst complex sec15 subunit. Demonstrated to react with rat and mouse tissues.|
|Immunogen:||Recombinant protein corresponding to full-length rat brain sec15 subunit|
|Purity:||Protein G purified|
|Tested Applications:||Western blotting, ELISA and Immunofluorescence microscopy.|
|Storage:||Store at -20C|
(left) Western blot analysis: 10 ug Rat brain and neuroendocrine PC12 cell lysates were resolved on a 8% SDS-polyacrylamide gel, transferred to nitrocellulose membrane and probed with 0.01ug/ml antibody.
(right) Immunofluorescence microcopy: Methanol-fixed neuroendocrine PC12 cells were stained with 0.1ug/ml antibody. For best visualization, the cells were incubated with the primary antibody at 4C overnight.
If you publish research with this product, please let us know so we can cite your paper.