LivePAR Lentivirus

LivePAR lentivirus preparation for expression of a genetically encoded poly-ADP-ribose (PAR) monitor in mammalian cells.

To overcome limitations of immunocytochemistry for poly-ADP-ribose (PAR) analysis in cells, the Sobol lab has developed a lentiviral vector for expression of a fluorescently tagged PAR-binding domain (PBD) fusion protein: LivePAR, a Live-cell imaging probe for poly(ADP-Ribosyl)ation (PAR). Studies from the Sobol lab document that the LivePAR probe is highly sensitive for PAR detection in cells, revealing the temporal dynamics of PAR formation and degradation by confocal fluorescent microscopy.

From the laboratory of Robert W. Sobol, PhD, University of South Alabama.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
ESA003
LivePAR Lentivirus
20uL In stock
Regular Price:$270.00
On Sale:
Specifications

Product Type: Virus
Biosafety Level: 2
Selection Gene: Cell selection using hygromycin, dose is cell dependent
Titer: ~2x10^12 VP/ml ( VP= virus particle) measured using the lentiviral rapid quantification kit from CellBioLabs. 1x10^5 U87MG cells were Infected with 1 tube virus and most of the cells were green after 48h as attached.
Serotype: HIV
Inoculation Conditions: Standard lentiviral transduction
Storage: -80C
Shipped: Dry Ice

Provider
From the laboratory of Robert W. Sobol, PhD, University of South Alabama.
References
  1. Koczor CA, Saville KM, Andrews JF, Clark J, Fang Q, Li J, Al-Rahahleh RQ, Ibrahim M, McClellan S, Makarov MV, Migaud ME, Sobol RW. Temporal dynamics of base excision/single-strand break repair protein complex assembly/ disassembly are modulated by the PARP/NAD+/SIRT6 axis. Cell Rep. 2021 Nov 2;37(5):109917. doi: 10.1016/j.celrep.2021.109917. PMID: 34731617

If you publish research with this product, please let us know so we can cite your paper.

Loading...