LivePAR lentivirus preparation for expression of a genetically encoded poly-ADP-ribose (PAR) monitor in mammalian cells.
To overcome limitations of immunocytochemistry for poly-ADP-ribose (PAR) analysis in cells, the Sobol lab has developed a lentiviral vector for expression of a fluorescently tagged PAR-binding domain (PBD) fusion protein: LivePAR, a Live-cell imaging probe for poly(ADP-Ribosyl)ation (PAR). Studies from the Sobol lab document that the LivePAR probe is highly sensitive for PAR detection in cells, revealing the temporal dynamics of PAR formation and degradation by confocal fluorescent microscopy.
From the laboratory of Robert W. Sobol, PhD, University of South Alabama.
|Selection Gene:||Cell selection using hygromycin, dose is cell dependent|
|Titer:||~2x10^12 VP/ml ( VP= virus particle) measured using the lentiviral rapid quantification kit from CellBioLabs. 1x10^5 U87MG cells were Infected with 1 tube virus and most of the cells were green after 48h as attached.|
|Inoculation Conditions:||Standard lentiviral transduction|
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