This purified mouse IgG1 monoclonal antibody was generated against purified stem cell marker TRA-1-60 antigen from the human embryonal carcinoma Tera-1.
TRA-1-60 is a pluripotent specific carbohydrate molecule present on the cell adhesion protein Podocalyxin. TRA-1-60 is highly expressed on normal embryonic stem cells. For the past forty years It has been used as the gold standard cell surface marker for all normal human embryonic stem cells. TRA-1-60 expression is lost upon differentiation of stem cells and is not present on normal differentiated human cells. Recently, numerous published studies have shown that TRA-1-60 is re-expressed in many kinds of human cancers: TRA-1-60 may be a cell marker of cancer cells with stem cell properties.
From the laboratory at CureMeta.
|Antigen:||TRA-1-60, Podocalyxin, gp200, GCTM-2, PCLP-1|
|Accession ID:||O00592, PODXL podocalyxin like [Homo sapiens (human)] Gene ID: 5420|
|Molecular Weight:||200-240 kDa|
|Purification Method:||Protein A|
|Method Used to Determine Concentration:||OD280|
|Buffer:||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2-7.4|
|Tested Applications:||Western Blotting (1:1000 with 1mg/ml), Immunohistochemistry/ImmunoFluorescence (1:200 with 1mg/ml), Immunoprecipitation, Flow cytometry|
|Storage:||Store at 4C short term. For long term storage, store at -20C. It is recommended that multiple freeze thaw cycles are avoided.|
TRA-1-60 in Human Embryonic Stem Cells:
TRA-1-60 in human cancer:
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