IgG Quantification ABC Kit (pan-specific)
IgG quantification ABC kit is an in vitro quantitative assay for the measurement of pan-species IgG in cell culture medium, serum or other biological samples.
Highlights:
- Pan-specific - Can be used to detect IgG from multiple species
- Mix&Read method - Does not require any washing step
- Includes all the materials for carrying out 96 tests in 96-well plate format. The test number can be increased to 384 if the users choose to use self-supplied 384-well plate.
IgG quantification ABC kit is an in vitro quantitative assay for the measurement of IgG in cell culture medium, serum or other biological samples. The assay reaction is a patent pending Mix&Read method and does not require any washing step. ABCK0001 is pan-specific and can be used to detect IgG from multiple species. ABC biotechnology has tested it on human, mouse and rabbit IgG. Other species has not been tested but the kit is expected to work too. ABCK0001 includes all the materials for carrying out 96 tests in 96-well plate format. The test number can be increased to 384 if the users choose to use self-supplied 384-well plate.
IgG quantification ABC kit is an in vitro quantitative assay for the measurement of pan-species IgG in cell culture medium, serum or other biological samples.
Highlights:
- Pan-specific - Can be used to detect IgG from multiple species
- Mix&Read method - Does not require any washing step
- Includes all the materials for carrying out 96 tests in 96-well plate format. The test number can be increased to 384 if the users choose to use self-supplied 384-well plate.
IgG quantification ABC kit is an in vitro quantitative assay for the measurement of IgG in cell culture medium, serum or other biological samples. The assay reaction is a patent pending Mix&Read method and does not require any washing step. ABCK0001 is pan-specific and can be used to detect IgG from multiple species. ABC biotechnology has tested it on human, mouse and rabbit IgG. Other species has not been tested but the kit is expected to work too. ABCK0001 includes all the materials for carrying out 96 tests in 96-well plate format. The test number can be increased to 384 if the users choose to use self-supplied 384-well plate.
| Product Type: | Kit |
| Name: | IgG Quantification ABC Kit (pan-specific) |
| Format: | 96-well plate |
| Components: |
White microplate (12x8-well strips) Reagent A (1 mL) 500X Reagent B (15 uL) General diluent (15 mL) Human IgG standard (20 uL, 0.5 mg/mL) Mouse IgG standard (20 uL, 0.5 mg/mL) |
| Tested Applications: | Measurement of pan-species IgG in cell culture medium, serum or other biological samples. |
| Storage: | -20C to +4C (see product manual) |
| Shipped: | Cold packs |
- The kit assay range is between 50 ng/ml to 50 ug/ml (depending on the IgG species and subclass). Samples with higher IgG concentration may generate low signals due to Hook Effect, therefore need to be pre-diluted to be used in the assay. For samples with unknown concentration range, it is recommended to test at several 10-fold dilutions.
- A general diluent is provided with the kit. However, for the most accurate result, it is recommended to use the same matrix to dilute the standards and samples. For example, in measuring the antibody expression from CHO cells, it is recommended to use antibody free CHO cell culture medium to dilute the standard.
- A standard curve is needed to quantitate the sample IgG concentration. IgG from the same species and of the same subclass should be used as standards for the most accurate result. Human and mouse IgG standards are provided with the kit. The standard curve may be omitted if the users only need to compare the relative concentrations among samples.
- This kit cannot be used for measuring IgG expression in serum containing medium due to the pan-specificity. For such applications, please use the human IgG specific kit ABCK0002 and mouse IgG specific kit ABCK0003.
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