Protein G purified Ovine IgG polyclonal antibody against RBD of SARS-CoV-2 spike protein.
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors that bind S1 are ACE2, DPP4, APN, etc. The spike protein is essential for both host specificity and viral infectivity. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
From the laboratory at Ruakura Technologies Ltd.
|Accession ID:||P0DTC2, YP_009724390|
|Molecular Weight:||26.7 kDa|
|Immunogen:||HisTag recombinant protein covering RBD domain of coronavirus spike protein|
|Species Immunized:||Ovine (sheep)|
|Epitope:||Corresponds to R319-G548-His6 (230aa)|
|Purification Method:||Ammonium sulphate precipitation/Protein G|
|Method Used to Determine Concentration:||BCA|
|Buffer:||30 mM Tris, 100 mM NaCl,1 mM PMSF, 0.02% sodium azide.|
|Tested Applications:||WB 1:5000, IF 1:2500, ELISA 1:1000|
|Concentration:||1mg/mL (200ug reconstituted in 200uL of water)|
|Storage:||Store at 4C. Once reconstituted, store at 4C short term. For long term storage, store at -20C. It is recommended that multiple freeze thaw cycles are avoided.|
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