Canine Myoblast Cell Line (Myok9)
This Myok9 Myoblast Cell Line is a normal genotyped, canine myoblast extracted from adult golden retriever/Labrador retriever mix dog vastus lateralis muscle. The cells have been immortalized with SV40 long T antigen.
Highlights:
- Developed from primary myoblast of a normal canine
- Immortalized myoblasts via SV40 viral transfection of the large T antigen into the primary muscle cells
- Expressed dystrophin and other muscle-specific proteins during differentiation (detected by mass spectrometry and western blotting)
- Useful for efficient new therapies development, before moving to pre-clinical studies
Myogenesis is the process of skeletal muscular tissue formation, particularly during an early embryo development during which myoblasts can either proliferate or differentiate into a myotube. Myoblasts are thus early descentants of muscle cells. Myok9, a canine myoblast cell line, was extracted from a normal genotyped adult canine (mixed breed) and could be useful for researchers studying or evaluating pottential future therapies for skeletal muscle disorders (such as Duschenne muscular dystrophy (DMD), myotubular myopathy, and others).
From the laboratory of Peter P. Nghiem, DVM, PhD, Texas A&M University.
This Myok9 Myoblast Cell Line is a normal genotyped, canine myoblast extracted from adult golden retriever/Labrador retriever mix dog vastus lateralis muscle. The cells have been immortalized with SV40 long T antigen.
Highlights:
- Developed from primary myoblast of a normal canine
- Immortalized myoblasts via SV40 viral transfection of the large T antigen into the primary muscle cells
- Expressed dystrophin and other muscle-specific proteins during differentiation (detected by mass spectrometry and western blotting)
- Useful for efficient new therapies development, before moving to pre-clinical studies
Myogenesis is the process of skeletal muscular tissue formation, particularly during an early embryo development during which myoblasts can either proliferate or differentiate into a myotube. Myoblasts are thus early descentants of muscle cells. Myok9, a canine myoblast cell line, was extracted from a normal genotyped adult canine (mixed breed) and could be useful for researchers studying or evaluating pottential future therapies for skeletal muscle disorders (such as Duschenne muscular dystrophy (DMD), myotubular myopathy, and others).
From the laboratory of Peter P. Nghiem, DVM, PhD, Texas A&M University.
| Product Type: | Cell Line |
| Name: | Canine Myoblast Cell Line (Myok9) |
| Cell Type: | Myoblast |
| Morphology: | Triangular shaped cells |
| Source: | Skeletal Muscle |
| Organism: | Dog |
| Biosafety Level: | BSL-1 |
| Growth Conditions: | DMEM, 20% FBS, 1% Penicillin/Streptomycin, 2% Chick embryo extract |
| Cryopreservation: | Complete media + 10% DMSO |
| Storage: | LN2 |
| Shipped: | Dry Ice |
- López SM, Balog-Alvarez C, Canessa EH, Hathout Y, Brown KJ, Vitha S, Bettis AK, Boehler J, Kornegay JN, Nghiem PP. Creation and characterization of an immortalized canine myoblast cell line: Myok9. Mamm Genome. 2020 Apr;31(3-4):95-109. doi: 10.1007/s00335-020-09833-5. Epub 2020 Apr 3. PMID: 32246189.
If you publish research with this product, please let us know so we can cite your paper.
