Ad CMV p53 - RSV eGFP expresses p53 via a CMV promoter in the E1 region of the virus and expresses eGFP from an RSV promoter in the E3 region.
Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
|Name:||Ad CMV p53 - RSV eGFP|
|Vector Information:||Recombinant adenovirus|
|Target Gene / Reporter(s):||p53, eGFP|
|Promoter:||CMV (p53), RSV (eGFP|
|Titer:||1X10^12 pts/mL, CsCl purified, RCA free and PFU titred|
|Storage:||Store at -80C. Avoid multiple freeze/thaw cycles.Virus is supplied at 1X10^12 particles/mL. We recommend thawing the virus at room temperature. For aliquoting, 0.5 mL screw cap vials are recommended. Other tubes do not seal tight enough and eventually the solution desiccates at -80 and the virus looses infectivity. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer in the original formulation. Do not store or thaw the virus on ice, -20 C or 4 C.|
|Shipped:||Frozen on Dry ice|
Cells should be infected when ~ 80% confluent and the normal growth media replaced with media containing only 2% serum. Media can be supplemented or replaced with normal growth media after 4 hrs if required. Wait at least 48 hrs prior to analysis by IHC, WB, ELISA etc.
If you publish research with this product, please let us know so we can cite your paper.