Autophagy Reporter H4 Cell Line (GFP-LC3/H4)
Human H4 neuroblastoma cell line expressing GFP-LC3 autophagy reporter.
LC3 is a mammalian homologue of yeast Atg8 (Aut7/Apg8) and serves as a marker protein for autophagosomes. The GFP-LC3/H4 cell line expresses GFP fused to LC3, allowing for measurement of autophagy. Monitoring of autophagy activity relies on microscopy visualization of fluorescent puncta, progressive degradation of the GFP. The number of fluorescent punctate structures per cell and the percentages of GFP positive cells can be used to assess autophagic flux.
From the laboratory of Junying Yuan, PhD, Harvard University.
Human H4 neuroblastoma cell line expressing GFP-LC3 autophagy reporter.
LC3 is a mammalian homologue of yeast Atg8 (Aut7/Apg8) and serves as a marker protein for autophagosomes. The GFP-LC3/H4 cell line expresses GFP fused to LC3, allowing for measurement of autophagy. Monitoring of autophagy activity relies on microscopy visualization of fluorescent puncta, progressive degradation of the GFP. The number of fluorescent punctate structures per cell and the percentages of GFP positive cells can be used to assess autophagic flux.
From the laboratory of Junying Yuan, PhD, Harvard University.
Specifications
| Product Type: | Cell Line |
| Name: | GFP-LC3/H4 |
| Cell Type: | Human H4 Neuroblastoma GFP-LC3 |
| Morphology: | Epithelial |
| Organism: | Human |
| Source: | Brain |
| Biosafety Level: | 2 |
| Growth Conditions: | DMEM, 10%FBS |
| Cryopreservation: | DMEM, 10% FBS, 8% DMSO |
| Storage: | LN |
| Shipped: | Dry Ice |
Provider
From the laboratory of Junying Yuan, PhD, Harvard University.
References
- Xu D, Zhao H, Jin M, Zhu H, Shan B, Geng J, Dziedzic SA, Amin P, Mifflin L, Naito MG, Najafov A, Xing J, Yan L, Liu J, Qin Y, Hu X, Wang H, Zhang M, Manuel VJ, Tan L, He Z, Sun ZJ, Lee VMY, Wagner G, Yuan J. Modulating TRADD to restore cellular homeostasis and inhibit apoptosis. Nature. 2020 Nov;587(7832):133-138. doi: 10.1038/s41586-020-2757-z. Epub 2020 Sep 23. PMID: 32968279.
- Xu Y, Yuan J, Lipinski MM. Live imaging and single-cell analysis reveal differential dynamics of autophagy and apoptosis. Autophagy. 2013 Sep;9(9):1418-30. doi: 10.4161/auto.25080. Epub 2013 May 29. PMID: 23748697
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