Highly versitle broad-range DNA ladder suitable for molecular biology research.
DNA ladders are essential research tools to every molecular biology laboratory. Commercially available ladders are offered as either narrow (more common) - or broad (less common)-range as they pertain to the range of DNA fragments that can be sized using these ladders. This broad-range ladder was developed using a set of three plasmids, which generate a set of 15 unique DNA fragments ranging from 100 bp to 10,000 bp. An essential feature of this ladder is that multiple fragments of the same size are generated (i.e., four fragments of 0.1 kbp, two fragments of 0.2 kbp and 0.5 kbp). This allows the ladder to maintain band intensity as fragments become smaller in size.
From the laboratory of Mikhail F. Alexeyev, PhD, University of South Alabama.
|Product Type:||Buffer or Chemical|
|Name:||Broad-Range DNA Ladder|
|Buffer:||15mM Tris, pH 8.0; 15.7mM K+; 3.5mM Mg+; 1.9mM EDTA; 0.0425% Bromphenol Blue; 8.5% glycerol; 0.17% SDS; 35ug/mL BSA|
|Marker Size:||100, 200, 300, 400, 500, 700, 800, 1000, 1500, 2000, 3000, 4000, 6000, 8000, 10000 bp|
|Tested Applications:||Ready-to-use: load 5uL per well for minigels or 10uL per well for midi/maxi gels|
|Storage:||Stable for at least 6 months at room temp. For long-term storage use -20C|
If you publish research with this product, please let us know so we can cite your paper.