Mouse Neuronal Cell Line (HT-22 BirAV5/3xBTE-Luc)

HT22[BirAV5/3xBTE-Luc] Adult mouse hippocampus-derived neuronal cell line; stably expresses biotin ligase BirA, and stably transfected with luciferase reporter with 3 basic transcription element (BTE) repeats.

From the laboratory of Robert J. Denver, PhD, University of Michigan.

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Mouse Neuronal Cell Line (HT-22 BirAV5/3xBTE-Luc)
1 Vial In stock
Regular Price:$580.00
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Product Type: Cell Line
Name: HT-22 [BirAV5/3xBTE-Luc]
Cell Type: Neuron
Morphology: Neuronal
Organism: Mouse
Source: Adult hippocampus; pSV40 immortalized
Biosafety Level: BSL1
Subculturing: Can be passaged with trypsin per standard protocols
Growth Conditions: High-glucose DMEM (Invitrogen) supplemented with 10% fetal bovine serum, penicillin G (100 U/ml) and streptomycin sulfate (100 _g/ml) under a humidified atmosphere of 5% CO2 at 37C. Maintain selection with 100 _g/ml zeocin. Please note that HT-22 cells are resistant to G-418.
Cryopreservation: DMEM growth medium with 10% DMSO
Comments: The HT22-pSV40-BirAV5 cell line was stably transfected with pGL4.23-3xBTE; this cell line reports (via luciferase) activation or repression mediated by the basic transcription element to which KLF9 and other KLF/Sp factors bind.
Storage: LN2
Shipped: Dry Ice

From the laboratory of Robert J. Denver, PhD, University of Michigan.
  1. Knoedler, J.R., Subramani, A. and Denver, R.J. (2017) The KrŸppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding. BMC Genomics 18:299. doi: 10.1186/s12864-017-3640-7 PMID:28407733.

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