Mouse Neuroblastoma Cell Line (N2a[TRb1])
![Mouse Neuroblastoma Cell Line (Neuro2A[TRb1])](https://www.kerafast.com/MediaStorage/Product/Images/Large/3570_2001202001281435690.jpg "Mouse Neuroblastoma Cell Line (N2a[TRb1])")
Neuro2A[TRβ1] mouse neuroblastoma cell line stably expressing human thyroid hormone receptor beta 1 (TRβ1).
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Neuro2A[TRβ1] mouse neuroblastoma cell line stably expressing human thyroid hormone receptor beta 1 (TRβ1).
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Specifications
| Product Type: | Cell Line |
| Name: | Neuro2A[TRb1] |
| Cell Type: | Neuron |
| Morphology: | Neuronal |
| Organism: | Mouse |
| Source: | Mouse neuroblastoma |
| Biosafety Level: | BSL1 |
| Growth Conditions: | DMEM/F12 supplemented with hygromycin B (500 ug/mL) and 10% fetal bovine serum stripped of thyroid hormone. |
| Subculturing: | Can be passaged with trypsin per standard protocols |
| Cryopreservation: | DMEM/F12 growth medium with 10% DMSO |
| Comments: | Cell line first created by Lebel et al. (1994) exhibits robust gene expression responses to thyroid hormone (T3), and differentiates (extends neurites) after treatment with T3 for 3-5 days. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
References
- Lebel JM, Dussault JH, Puymirat J. Overexpression of the beta 1 thyroid receptor induces differentiation in neuro-2a cells. Proc Natl Acad Sci U S A. 1994;91(7):2644-2648; Kyono, Y., Subramani, A., Ramadoss, P., Hollenberg, A.N., Bonett, R.M. and Denver, R.J. (2016) Liganded thyroid hormone receptors transactivate the DNA methyltransferase 3a gene in mouse neuronal cells. Endocrinology 157:3647-3657 doi: 10.1210/en.2015-1529 PMID:27387481
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