Mouse Neuronal Cell Line (HT-22 FLBIO-rKLF9/SV40-BirAV5)

HT22-FLBIO-rKLF9/BirAV5 is an adult mouse hippocampus-derived neuronal cell line; engineered to express the biotin ligase BirAV5 and rat Kruppel-like factor 9 (KLF9; becomes biotinylated in vivo).

From the laboratory of Robert J. Denver, PhD, University of Michigan.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
Mouse Neuronal Cell Line (HT-22 FLBIO-rKLF9/SV40-BirAV5)
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Product Type: Cell Line
Name: HT22-FLBIO-rKLF9/SV40-BirAV5
Cell Type: Neuron
Accession ID: Q13886
Organism: Mouse
Source: Adult hippocampus; pSV40 immortalized
Biosafety Level: BSL1
Subculturing: Can be passaged with trypsin per standard protocols
Growth Conditions: High-glucose DMEM (Invitrogen) supplemented with 10% fetal bovine serum, penicillin G (100 U/ml) and streptomycin sulfate (100 ug/ml) under a humidified atmosphere of 5% CO2 at 37C. Maintain selection with 100 ug/ml zeocin plus 1 ug/ml puromycin. Please note that HT-22 cells are resistant to G-418.
Cryopreservation: DMEM growth medium with 10% DMSO
Comments: This cell line expresses biotinylated KLF9 for chromatin precipitation, protein-protein interactions, etc.
Storage: LN2
Shipped: Dry Ice

From the laboratory of Robert J. Denver, PhD, University of Michigan.
  1. Knoedler, J.R., Subramani, A. and Denver, R.J. (2017) The Kruppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding. BMC Genomics 18:299. doi: 10.1186/s12864-017-3640-7 PMID:28407733.

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