Mouse Neuronal Cell Line (HT-22 BLRP-hTRb/pSV40-BirAV5)
HT22-BLRP-hTRb/pSV40-BirAV5 is an adult mouse hippocampus-derived neuronal cell line; engineered to express the biotin ligase BirAV5 and human thyroid hormone receptor beta BIO fusion protein (becomes biotinylated in vivo).
From the laboratory of Robert J. Denver, PhD, University of Michigan.
HT22-BLRP-hTRb/pSV40-BirAV5 is an adult mouse hippocampus-derived neuronal cell line; engineered to express the biotin ligase BirAV5 and human thyroid hormone receptor beta BIO fusion protein (becomes biotinylated in vivo).
From the laboratory of Robert J. Denver, PhD, University of Michigan.
Specifications
| Product Type: | Cell Line |
| Name: | HT22-BLRP-hTRb/pSV40-BirAV5 |
| Cell Type: | Neuron |
| Organism: | Mouse |
| Source: | Adult hippocampus; pSV40 immortalized |
| Biosafety Level: | BSL1 |
| Subculturing: | Can be passaged with trypsin per standard protocols |
| Growth Conditions: | High-glucose DMEM (Invitrogen) supplemented with 10% fetal bovine serum, penicillin G (100 U/ml) and streptomycin sulfate (100 ug/ml) under a humidified atmosphere of 5% CO2 at 37C. Maintain selection with 100 ug/ml zeocin plus 1 ug/ml puromycin. Please note that HT-22 cells are resistant to G-418. |
| Cryopreservation: | DMEM growth medium with 10% DMSO |
| Comments: | This cell line expresses biotinylated TRbeta for chromatin precipitation sequencing, protein-protein interactions, etc. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Robert J. Denver, PhD, University of Michigan.
References
- Knoedler, J.R., Subramani, A. and Denver, R.J. (2017) The Kruppel-like factor 9 cistrome in mouse hippocampal neurons reveals predominant transcriptional repression via proximal promoter binding. BMC Genomics 18:299. doi: 10.1186/s12864-017-3640-7 PMID:28407733.
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