DLEC is a continuous adherent cell line derived from early embryos of the European sea bass Dicentrarchus labrax.
The European bass (Dicentrarchus labrax) is a primarily ocean-going fish native to the waters off Europe's western and southern and Africa's northern coasts.
From the laboratory of Francesco Buonocore, PhD and Giuseppe Scapigliati, PhD, University of Tuscia.
|Product Type:||Cell Line|
|Cell Type:||Embryonic cell line|
|Source:||European sea bass - Early embryo|
|Organism:||Dicentrarchus labrax (European sea bass)|
|Biosafety Level:||BSL 1|
|Subculturing:||Split every ~72 hours, when semi-confluent. Note that the cells are quite sensitive to trypsinization. Use minimal amounts of trypsin-EDTA after two washes with PBS. Monitor the trypsinization at the microscope, when cells became rounded detach by pipetting with complete L-15 and split 1:3.|
|Growth Conditions:||Grow at 22 °C in sealed flasks (or avoid CO2) with Liebovitz's L15 medium containing Pen-Strep and 10% heat-inactivated FBS, adjust the osmolarity of all solutions by adding 5 ul/ml of 3M NaCl.|
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