Ad CMV Cre - RSV eGFP expresses Cre via a CMV promoter in the E1 region of the virus and expresses eGFP from an RSV promoter in the E3 region.
Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.
|Ad CMV Cre - RSV eGFP
|Target Gene / Reporter(s):
|CMV (Cre), RSV (eGFP)
|1X10^12 pts/mL, CsCl purified, RCA free and PFU titred
|Store at -80C. Avoid multiple freeze/thaw cycles.Virus is supplied at 1X10^12 particles/mL. We recommend thawing the virus at room temperature. For aliquoting, 0.5 mL screw cap vials are recommended. Other tubes do not seal tight enough and eventually the solution desiccates at -80 and the virus looses infectivity. Aliquots of less than 50uL are unstable and should be avoided. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer in the original formulation. Do not store or thaw the virus on ice, -20 C or 4 C.
|Frozen on Dry ice
Cells should be infected when ~ 80% confluent and the normal growth media replaced with media containing only 2% serum. Media can be supplemented or replaced with normal growth media after 4 hrs if required. Wait at least 48 hrs prior to analysis by IHC, WB, ELISA etc.
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