Ad CMV Cre

Ad Cre expresses Cre under control of a CMV promoter.

Ready-made control adenoviruses (Ad) are generally in stock and can ship anytime. These particles are CsCl purified and supplied in 100 uL aliquots at 1X10^12 pts/mL. These preps are RCA free and PFU titered.

Catalog Number Product Size AVAILABILITY Price Qty
FVQ004
Ad CMV Cre
100uL (1x10^12 PFU/mL) In stock
Regular Price:$475.00
Specifications

Product Type: Virus
Name: Ad CMV Cre
Biosafety Level: BSL-2
Vector Information: Recombinant adenovirus
Target Gene / Reporter(s): Cre
Promoter: CMV
Virus: Adenovirus
Titer: 1X10^12 pts/mL, CsCl purified, RCA free and PFU titred
Storage: Store at -80C. Avoid multiple freeze/thaw cycles.Virus is supplied at 1X10^12 particles/mL. We recommend thawing the virus at room temperature. For aliquoting, 0.5 mL screw cap vials are recommended. Other tubes do not seal tight enough and eventually the solution desiccates at -80 and the virus looses infectivity. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer in the original formulation. Do not store or thaw the virus on ice, -20 C or 4 C.
Shipped: Frozen on Dry ice

Comments

Ad CMV Cre #17187

Cells should be infected when ~ 80% confluent and the normal growth media replaced with media containing only 2% serum. Media can be supplemented or replaced with normal growth media after 4 hrs if required. Wait at least 48 hrs prior to analysis by IHC, WB, ELISA etc.

References
  1. Lua I, Li Y, Pappoe LS, Asahina K. Myofibroblastic Conversion and Regeneration of Mesothelial Cells in Peritoneal and Liver Fibrosis. Am J Pathol. 2015 Dec;185(12):3258-73. View Article
  2. Li Y, Lua I, French SW, Asahina K. Role of TGF-β signaling in differentiation of mesothelial cells to vitamin A-poor hepatic stellate cells in liver fibrosis. Am J Physiol Gastrointest Liver Physiol. 2016 Feb 15;310(4):G262-72. View Article
  3. Anderson RD, Haskell RE, Xia H, Roessler BJ, and Davidson BL. A Simple Method for the Rapid Generation of Recombinant Adenovirus Vectors. Gene Therapy. (2000) 7, 1034-1038.
  4. Hennigan RF, Fletcher JS, Guard S, Ratner N. Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins. Sci Signal. 2019 Apr 23;12(578). pii: eaau8749. View Article

If you publish research with this product, please let us know so we can cite your paper.

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