Anti-RILP [BBRILP 36.1] Antibody
![Anti-RILP [BBRILP 36.1] Antibody](https://www.kerafast.com/MediaStorage/Product/Images/Large/1046_2001202001263210450.jpg "Anti-RILP [BBRILP 36.1] Antibody")
This mouse IgG2ak monoclonal antibody was raised against bacterially expressed C-terminal half of human (Rab interacting lysosomal protein) RILP and recognizes human RILP.
Highlights:
- Reacts with human RILP
- Suitable for Western Blot, Immunofluorescence and Immunocytochemistry applications
Rabs are a large family of small GTPases best known for their involvement in intracellular membrane trafficking. When activated, Rabs recruit their effectors to membrane compartments to exert diverse functions such as sorting of cargo into budding vesicles, vesicle movement along the cytoskeleton and tethering and fusion to target membranes. Functional impairments of Rabs or their accessory proteins are manifested in a range of diseases including neurological disorders and diabetes as well as in infectious diseases . RILP (Rab interacting lysosomal protein) was first identified as a Rab7 effector. By binding to the dynein motor complexes, RILP promotes microtubule-mediated transport of Rab7-positive late endosomes and lysosomes away from the cell periphery and toward the nucleus. Interestingly, RILP also acts as an effector for the predominantly Golgi-localized Rab34. The Rab34/RILP association, which presumably is taking place at the Golgi appears to control the distant lysosomal distribution. Important note: several lines of publicly available data indicate low and very restricted expression of RILP in immortalized cell lines as well as in tissues.
From a laboratory at Agency for Science, Technology and Research (A*STAR).
This mouse IgG2ak monoclonal antibody was raised against bacterially expressed C-terminal half of human (Rab interacting lysosomal protein) RILP and recognizes human RILP.
Highlights:
- Reacts with human RILP
- Suitable for Western Blot, Immunofluorescence and Immunocytochemistry applications
Rabs are a large family of small GTPases best known for their involvement in intracellular membrane trafficking. When activated, Rabs recruit their effectors to membrane compartments to exert diverse functions such as sorting of cargo into budding vesicles, vesicle movement along the cytoskeleton and tethering and fusion to target membranes. Functional impairments of Rabs or their accessory proteins are manifested in a range of diseases including neurological disorders and diabetes as well as in infectious diseases . RILP (Rab interacting lysosomal protein) was first identified as a Rab7 effector. By binding to the dynein motor complexes, RILP promotes microtubule-mediated transport of Rab7-positive late endosomes and lysosomes away from the cell periphery and toward the nucleus. Interestingly, RILP also acts as an effector for the predominantly Golgi-localized Rab34. The Rab34/RILP association, which presumably is taking place at the Golgi appears to control the distant lysosomal distribution. Important note: several lines of publicly available data indicate low and very restricted expression of RILP in immortalized cell lines as well as in tissues.
From a laboratory at Agency for Science, Technology and Research (A*STAR).
| Product Type: | Antibody |
| Accession ID: | Q96NA2, 83547 |
| Antigen: | RILP |
| Molecular Weight: | 44 kDa |
| Isotype: | IgG2ak |
| Clonality: | Monoclonal |
| Clone Name: | BBRILP 36.1 |
| Reactivity: | Human |
| Immunogen: | Bacterially expressed C-terminal half of human RILP. |
| Species Immunized: | Mouse |
| Purification Method: | Protein G |
| Buffer: | PBS, 0.05% (w/v) Sodium Azide |
| Tested Applications: | Western Blot and Immunofluorescence/Immunocytochemistry |
| Storage: | -20C |
| Shipped: | Cold Packs |
- Zhen, Y., H. Stenmark. 2015. Cellular functions of Rab GTPases at a glance. J Cell Sci. 128(17):3171-6.
- Hutagalung, A.H., P.J. Novick. 2009. Role of Rab GTPases in membrane traffic and cell physiology. Physiol Rev. 91(1):119-49.
- Cantalupo, G., P. Alifano, V. Roberti, C.B Bruni, C. Bucci. 2001. Rab-interacting lysosomal protein (RILP): the Rab7 effector required for transport to lysosomes. EMBO J. 20(4): 683-93.
- Jordens, I., M. Fernandez-Borja, M. Marsman, S. Dusseljee, L. Janssen, J. Calafat, H. Janssen, R. Wubbolts, J. Neefjes. 2001. The Rab7 effector protein RILP controls lysosomal transport by inducing the recruitment of dynein-dynactin motors. Curr Biol. 11(21):1680-5.
- Christopher, S. 2018. This Is the End: Regulation of Rab7 Nucleotide Binding in Endolysosomal Trafficking and Autophagy. Front Cell Dev Biol. 6:129. doi: 10.3389/fcell.2018.00129. eCollection 2018.
- Wang, T., W. Hong. 2002. Interorganellar regulation of lysosome positioning by the Golgi apparatus through Rab34 interaction with Rab-interacting lysosomal protein. Mol Biol Cell. 13(12):4317-32.
- Uhlén, M., et al. 2015. Tissue-based map of the human proteome. Science. 347(6220):1260419. PubMed: 25613900.
- Koscielny, G., et al. 2014. The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data. Nucleic Acids Res. 42(Database issue):D802-9.
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