Recombinant human ACE2-NN-IgG Fc (LALA)-Fusion Protein expressed in HEK293 cells.
ACE2 acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. ACE2-NN is its inactivated mutant form (H374N and H378N mutations).
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|Alternative Name(s):||ACE2-NN-Fc chimera; ACE2-NN (Fc tag); ACEH; Metalloprotease MPROT15|
|Fc domain:||Human IgG1 containing silencing LALA mutations|
|Extinction Coefficient:||376360 M-1 cm-1 (calculation performed as in Pace et al, 1995)|
|Molecular Weight:||190493.08 Da|
|Amino Acid Sequence:||QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKL QLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEV GKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLM NAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQG FWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHNEMGNIQYDMAYAAQPFLLRNGANEGFHE AVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEM KREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADGGGGSGGGGSDKTHTCPP CPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESN GQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK|
|Buffer:||PBS, 0.02% Proclin 300|
|Activity:||ACE2 acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. ACE2-NN is its inactivated mutan|
|Storage:||Recommended storage at +4C for up to 1 month. For longer term storage store at -20C or -80C in appropriate sized aliquots.|
ELISA: ACE2 Fc fusion protein binding to immobilized SARS-CoV-2 RBD Fc fusion protein. 96-well plate was coated with the SARS-CoV-2 RBD Fc fusion protein at 5 ug/ml. The ACE2 Fc fusion protein (Kf-Pr00439) were titrated on a 3-fold serial dilution starting at 10 ug/ml.
RBD Fc binding to immobilized ACE2 Fc fusion proteins. 96-well plates were coated with the ACE2 Fc fusion proteins (Kf-Pr00439 and Kf-Pr00441) at 5 ug/ml. The SARS-CoV-2 RBD Fc fusion protein conjugated to HRP was titrated on a 3-fold serial dilution starting at 10 ug/ml.
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