B6/129 Macrophage Cell Line

Macrophage cell line isolated from bone marrow in B6/129 mice after immortalization with a retrovirus (J2) containing the myc and v-raf oncogenes.

B6/129 mouse line is a cross between C57BL/6 females (B6) and 129P3 males (129P). Potential uses for these mice include hybrid vigor, as a background for deleterious mutations, to create or enhance expression of polygenic diseases, to determine the mode of inheritance, to provide physiological buffering (present a broader array of responses to various stresses) and as approximate controls for some genetically engineered strains.

From the laboratory of Howard A. Young, PhD, National Cancer Institute/NIH.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
B6/129 Macrophage Cell Line
1 vial In stock
Regular Price:$580.00
On Sale:

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Product Type: Cell Line
Name: B6/129
Cell Type: Macrophage
Morphology: Adherent
Source: Bone Marrow
Organism: Mouse (B6/129)
Biosafety Level: BSL2
Growth Conditions: DMEM + 10% FBS + Glutamine
Cryopreservation: 90% FBS + 10% DMSO
Storage: Liquid Nitrogen
Shipped: Dry Ice

From the laboratory of Howard A. Young, PhD, National Cancer Institute/NIH.
The line is not producing any murine Type C retroviruses as it was derived with a replication defective packaging cell line.
  1. Blasi E, Radzioch D, Merletti L, Varesio L. Generation of macrophage cell line from fresh bone marrow cells with a myc/raf recombinant retrovirus. Cancer Biochem Biophys. 1989;10(4):303-317.
  2. Blasi E, Mathieson BJ, Varesio L, Cleveland JL, Borchert PA, Rapp UR. Selective immortalization of murine macrophages from fresh bone marrow by a raf/myc recombinant murine retrovirus. Nature. 1985;318(6047):667-670.
  3. Xiao C, Wang RH, Lahusen TJ, et al. Progression of chronic liver inflammation and fibrosis driven by activation of c-JUN signaling in Sirt6 mutant mice. J Biol Chem. 2012;287(50):41903-41913.

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