MetalloBuffer BufferTM Kit pZn 13-9

MetalloBufferTM Buffer Kit pZn 13-9 is a set of 2x 10mM HEPES 10 mM NTA 125 mM NaCl pH 7.5 buffers that when mixed together as directed provide fixed, low free zinc concentrations between 0.1 pM (pZn = 13) and 1.0 nM (pZn = 9) at 25C.

To calibrate the response of sensors such as fluorescent chemosensors against low concentrations of free (mobile, exchangeable) zinc ion near physiological pH is difficult, because of contaminating metal ions, the propensity of zinc ions to bind to ligands or glass surfaces, and the tendency of zinc ions to hydrolyse and precipitate near neutral pH. MetalloBuffersTM minimize these issues by effectively buffering the free zinc ion levels in a manner analogous to pH buffers. MetalloBufferTM pZn levels are corrected for ionic strength and temperature effects.

Read plog post from Dr. Richard Thompson at Pokegama Technologies, Improving Zinc Ion Quantitation with Unique Biosensor Proteins and Buffers.

Catalog Number Product DataSheet Size AVAILABILITY Price Qty
KPG002
MetalloBuffer BufferTM Kit pZn 13-9
1 Set In stock
Regular Price:$350.00
On Sale:
Specifications

Product Type: Buffer or Chemical
Name: MetalloBufferTM Buffer Kit pZn 13-9
Product MPN: M-0005
Format: Set of bottles containing differing total zinc concentrations
Tested Applications: Calibration of fluorescent zinc sensors
Solubility: Aqeuous solutions
Storage: Room Temperature
Shipped: Room Temperature

Data

Shown is a ratiometric calibration curve of a fluorescent-labeled apocarbonic anhydrase II with excitation at 365 and 546 nm, and emission at 617 nm.

Comments
Buffers are provided as 2x solutions: e.g., to get final concentrations of buffer, buffer is mixed 1:1 v/v with distilled water. Please see enclosed instructions.
References
  1. R.A. Bozym, T. Hurst, N. Westerberg, A.V. Stoddard, C.A. Fierke, C.J. Frederickson, R.B. Thompson, "Determination of zinc using carbonic anhydrase-based fluorescence biosensors" in Methods in Enzymology: Fluorescence Spectroscopy Vol. 450 (L. Brand and M.L. Johnson, editors) New York: Elsevier, pp 279-301 (2008). PMID 19152866.
  2. W. Stumm and J.J. Morgan, Aquatic Chemistry, 3rd Edition (Wiley-Interscience) 1996.

If you publish research with this product, please let us know so we can cite your paper.

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