Human embryonic kidney (HEK293) cells that stably express Interferon Regulatory Factor 3 (IRF-3) tagged by a Venus fluorescent protein.
Interferon regulatory factors (IRF) are proteins that regulate transcription of interferons (see regulation of gene expression). IRF3 is a key transcription factor to activate the expression of interferons. IRF3 contains several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain, and several regulatory phosphorylation sites. IRF3 plays an important role in the innate immunity against viral infection. IRF3 has been shown to interact with IRF7. HEK293-IRF3 was established with a stale expression of IRF3 and used in studies of the interferon induction pathway.
From the laboratory of Yanjin Zhang, PhD, University of Maryland, College Park.
|Product Type:||Cell Line|
|Cell Type:||Human embryonic kidney cells|
|Morphology:||Monolayer cells, Epithelial|
|Biosafety Level:||BSL 2|
|Subculturing:||Adherent, 1:3 dilution|
|Growth Conditions:||DMEM with 10% FBS, 1x Penicillin-Streptomycin-Glutamine, 1x sodium pyruvate, and plasmocin of 1 ug/ml|
|Cryopreservation:||Complete growth medium supplemented with 10% DMSO|
|Comments:||HEK293 cells stably expressing VenusC1-IRF-3 (HEK293-IRF-3) were established by transfection of the cells with VenusC1-IRF-3 and selection under the antibiotic G418.|
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