SuperTopFlash (STF) HEK293 Cell Line

SuperTop (STF) HEK293 is a luciferase reporter cell line that responds to canonical Wnt signaling by expressing firefly luciferase.

Highlights:

  • Cells have been shown to respond to activators of the canonical Wnt pathway in mice, inducing luciferase activity by 1000x

This is a stable cell line that was made from HEK/293 cells and is G418 resistant. The cell line, which contains a luciferase reporter under control of 7 LEF/TCF binding sites, utilizes the Super TOPFlash system. It responds to activation of the canonical Wnt signaling pathway by increasing expression of firefly luciferase.

From the laboratory of Jeremy Nathans, MD, PhD, The Johns Hopkins University.

Catalog Number Product Size AVAILABILITY Price Qty
EJH014
SuperTopFlash (STF) HEK293 Cell Line
1 Vial 3-5 weeks
Regular Price:$499.00
Specifications

Product Type: Cell Line
Name: SuperTopFlash (STF) Cell Line
Cell Type: HEK293-derived
Organism: Human
Morphology: Attached epithelial
Source: Kidney
Biosafety Level: BSL 2 (Cells contain adenovirus viral DNA sequences)
Growth Conditions: DMEM/F12, 10% FBS, 200 ug/ml G418
Subculturing: Robust
Cryopreservation: DMEM/F12, 50% serum, 10% DMSO
Age: Fetus
Comments: The cells are G418 resistant and should be maintained in 200 ug/ml G418.
Storage: LN2
Shipped: Dry Ice

Provider
From the laboratory of Jeremy Nathans, MD, PhD, The Johns Hopkins University.
References
  1. Xu Q, Wang Y, Dabdoub A, Smallwood PM, Williams J, Woods C, Kelley MW, Jiang L, Tasman W, Zhang K, Nathans J. Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair. Cell. 2004 Mar 19;116(6):883-95.

If you publish research with this product, please let us know so we can cite your paper.

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