SuperTopFlash (STF) HEK293 Cell Line
SuperTop (STF) HEK293 is a luciferase reporter cell line that responds to canonical Wnt signaling by expressing firefly luciferase.
Highlights:
- Cells have been shown to respond to activators of the canonical Wnt pathway in mice, inducing luciferase activity by 1000x
This is a stable cell line that was made from HEK/293 cells and is G418 resistant. The cell line, which contains a luciferase reporter under control of 7 LEF/TCF binding sites, utilizes the Super TOPFlash system. It responds to activation of the canonical Wnt signaling pathway by increasing expression of firefly luciferase.
From the laboratory of Jeremy Nathans, MD, PhD, The Johns Hopkins University.
SuperTop (STF) HEK293 is a luciferase reporter cell line that responds to canonical Wnt signaling by expressing firefly luciferase.
Highlights:
- Cells have been shown to respond to activators of the canonical Wnt pathway in mice, inducing luciferase activity by 1000x
This is a stable cell line that was made from HEK/293 cells and is G418 resistant. The cell line, which contains a luciferase reporter under control of 7 LEF/TCF binding sites, utilizes the Super TOPFlash system. It responds to activation of the canonical Wnt signaling pathway by increasing expression of firefly luciferase.
From the laboratory of Jeremy Nathans, MD, PhD, The Johns Hopkins University.
Specifications
| Product Type: | Cell Line |
| Name: | SuperTopFlash (STF) Cell Line |
| Cell Type: | HEK293-derived |
| Morphology: | Attached epithelial |
| Organism: | Human |
| Source: | Kidney |
| Biosafety Level: | BSL 2 (Cells contain adenovirus viral DNA sequences) |
| Growth Conditions: | DMEM/F12, 10% FBS, 200 ug/ml G418 |
| Subculturing: | Robust |
| Cryopreservation: | DMEM/F12, 50% serum, 10% DMSO |
| Age: | Fetus |
| Comments: | The cells are G418 resistant and should be maintained in 200 ug/ml G418. |
| Storage: | LN2 |
| Shipped: | Dry Ice |
Provider
From the laboratory of Jeremy Nathans, MD, PhD, The Johns Hopkins University.
References
- Xu Q, Wang Y, Dabdoub A, Smallwood PM, Williams J, Woods C, Kelley MW, Jiang L, Tasman W, Zhang K, Nathans J. Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair. Cell. 2004 Mar 19;116(6):883-95.
- Jergova S, Perez C, Imperial JS, Gajavelli S, Jain A, Abin A, Olivera BM, Sagen J. Cannabinoid receptor agonists from Conus venoms alleviate pain-related behavior in rats. Pharmacol Biochem Behav. 2021 Mar 25;205:173182. View Article
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