SuperTop (STF) HEK293 is a luciferase reporter cell line that responds to canonical Wnt signaling by expressing firefly luciferase.
This is a stable cell line that was made from HEK/293 cells and is G418 resistant. The cell line, which contains a luciferase reporter under control of 7 LEF/TCF binding sites, utilizes the Super TOPFlash system. It responds to activation of the canonical Wnt signaling pathway by increasing expression of firefly luciferase.
From the laboratory of Jeremy Nathans, MD, PhD, The Johns Hopkins University.
|Product Type:||Cell Line|
|Name:||SuperTopFlash (STF) Cell Line|
|Biosafety Level:||BSL 2 (Cells contain adenovirus viral DNA sequences)|
|Growth Conditions:||DMEM/F12, 10% FBS, 200 ug/ml G418|
|Cryopreservation:||DMEM/F12, 50% serum, 10% DMSO|
|Comments:||The cells are G418 resistant and should be maintained in 200 ug/ml G418.|
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