Cholesterol 22-hydroxylase (CYP90B27)

CYP90B27 is a cytochrome P450 enzyme that catalyzes the hydroxylation of cholesterol at position C-22 to form 22-hydroxycholesterol. CYP90B27 can also hydroxylate 27(25R)-hydroxycholesterol and 7?-hydroxycholesterol, also at position C-22.

From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.

Catalog Number Product Size AVAILABILITY Price Qty
EDD011
Cholesterol 22-hydroxylase (CYP90B27)
500uL 1-2 weeks
Regular Price:$250.00
Specifications

Product Type: Protein
Name: 22-Hydroxycholesterol 26-hydroxylase/oxidase
Accession ID: KJ869252
Source: Sf9 Cells
Molecular Weight: 54,919
Buffer: 100 mM tricine pH 7.5/ 5 mM thioglycolic acid
Comments: Requires NADPH
Storage: -80C
Shipped: Dry Ice

Provider
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
References
  1. Augustin, M. M., D. R. Ruzicka, A. K. Shukla, J. M. Augustin, C. M. Starks, M. O'Neil-Johnson, M. R. McKain, B. S. Evans, M. D. Barrett, A. Smithson, G. K. Wong, M. K. Deyholos, P. P. Edger, J. C. Pires, J. H. Leebens-Mack, D. A. Mann and T. M. Kutchan (2015). "Elucidating steroid alkaloid biosynthesis in Veratrum californicum: production of verazine in Sf9 cells." Plant J 82(6): 991-1003.

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