CYP90B27 is a cytochrome P450 enzyme that catalyzes the hydroxylation of cholesterol at position C-22 to form 22-hydroxycholesterol. CYP90B27 can also hydroxylate 27(25R)-hydroxycholesterol and 7?-hydroxycholesterol, also at position C-22.
From the laboratory of Toni M. Kutchan, PhD, and Megan Augustin, Donald Danforth Plant Science Center.
|Buffer:||100 mM tricine pH 7.5/ 5 mM thioglycolic acid|
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