Anti-p53 [Pab240] Antibody

This mouse IgG1k monoclonal antibody recognizes mammalian and chicken p53 and was generated against a gel purified p53-b-galactosidase fusion protein containing a sequence corresponding to amino acids 14-389 of p53 (derived from the pSV53C p53 cDNA clone).

p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. Activated p53 binds DNA and activates expression of several genes leading to unregulated cellular proliferation.

From a laboratory at Cancer Research Technology.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EW0005
Anti-p53 [Pab240] Antibody
0.1mg (100uL at 1mg/mL) 2-3 weeks
Regular Price:$356.00
Specifications

Product Type: Antibody
Name: Anti-p53 (Pab 240) monoclonal antibody
Host: Balb/c
Isotype: IgG1k
Clonality: Monoclonal
Clone Name: Pab240
Specificity: Mammals and chicken
Immunogen: A gel purified p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone)
Format: Liquid
Purity: Protein G purified
Tested Applications: FACS, Immunoprecipitation, Paraffin embedded, WB
Concentration: 1mg/mL
Amount: 100uL
Storage: Store at 4C
Shipped: Cold packss

Provider
From a laboratory at Cancer Research Technology.
Comments

Detection of p53 using PAb 240: 50% colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissue are negative.

References
  1. Iggo R, Gatter K, Bartek J, Lane D, Harris AL. Increased expression of mutant forms of p53 oncogene in primary lung cancer. 1990. Lancet. 335:675-9. PMID: 1969059
  2. Gannon JV, Greaves R, Iggo R, Lane DP. Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form. 1990. EMBO J. 9:1595-602. PMID: 1691710
  3. Bartek J, Bartkova J, Vojtesek B, Staskova Z, Lukas J, Rejthar A, Kovarik J, Midgley CA, Gannon JV, Lane DP. Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies. 1991. Oncogene. 6:1699-703. PMID: 1923535
  4. Said JW, Barrera R, Shintaku IP, Nakamura H, Koeffler HP. Immunohistochemical analysis of p53 expression in malignant lymphomas. 1992. Am. J Pathol. 141:1343-8. PMID: 1466398
  5. Walker RA, Dearing SJ, Lane DP, Varley JM. Expression of p53 protein in infiltrating and in-situ breast carcinomas. 1991. J Pathol. 165:203-11. PMID: 1684809

If you publish research with this product, please let us know so we can cite your paper.

Loading...