This mouse IgG1k monoclonal antibody recognizes mammalian and chicken p53 and was generated against a gel purified p53-b-galactosidase fusion protein containing a sequence corresponding to amino acids 14-389 of p53 (derived from the pSV53C p53 cDNA clone).
p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. Activated p53 binds DNA and activates expression of several genes leading to unregulated cellular proliferation.
From a laboratory at Cancer Research Technology.
Part of The Investigator's Annexe program.
|Name:||Anti-p53 (Pab 240) monoclonal antibody|
|Specificity:||Mammals and chicken|
|Immunogen:||A gel purified p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone)|
|Purity:||Protein G purified|
|Tested Applications:||FACS, Immunoprecipitation, Paraffin embedded, WB|
|Storage:||Store at 4C|
Detection of p53 using PAb 240: 50% colon cancer sections are positive, 30% of breast cancer sections and 70% of lung cancer sections. Normal and premalignant tissue are negative.
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