Anti-E2F3 [LLF3#2G2] Antibody

This mouse monoclonal antibody was generated against human E2F3 (aa 1-244, 6X His-tagged) and recognizes both isoforms of human and mouse E2F3, E2F3a and E2F3b.

E2F3 is a winged helix transcription factor important for regulating the expression of genes encoding proteins required for cell cycle entry, DNA synthesis and mitosis and is an important target of pRB, a tumor suppressor protein. E2F3 can function in complexes that either repress or activate transcription. Studies using E2f3 mutant mice also show that E2F3 is important for cell cycle regulation, heart development and disease, cell migration and cancer. In addition in some human cancers E2F3 is over-expressed.

From a laboratory at Massachusetts Institute of Technology.

The Investigator's Annexe Part of The Investigator's Annexe program.

Catalog Number Product Size AVAILABILITY Price Qty
EC0001
Anti-E2F3 [LLF3#2G2] Antibody
100ug In stock
Regular Price:$328.00
Specifications

Product Type: Antibody
Name: E2F3 monoclonal antibody
Host: Mouse
Isotype: IgG
Clonality: Monoclonal
Clone Name: LLF3#2G2
Specificity: human, mouse
Immunogen: Antibody was raised against 6x His-tagged human E2F3 (aa 1 - 244)
Format: Liquid
Purity: Protein G purified
Tested Applications: ELISA, Western Blot, ChIP
Concentration: 1mg/mL
Amount: 100uL
Storage: +4C or -20C (avoid repeated freeze / thaw cycles)
Shipped: Cold packs

Data

Western Blot

Protein extracts were derived from mouse embryo fibroblasts (MEF)s in wild-type and E2f3a and E2f3b knockout littermates and probed with E2f3 monoclonal antibody (from LLF3#2G2).

Provider
References
  1. Danielian PS, Friesenhahn LB, Faust AM, West JC, Caron AM, Bronson RT, Lees, JA (2008) E2f3a and E2f3b make overlapping but different contributions to total E2f3 activity. Oncogene. 27(51): 6561 - 70
  2. Parisi T, Yuan TL, Faust AM, Caron AM, Bronson R, Lees JA (2007) Selective requirements for E2f3 in the development and tumorigenecity of Rb-deficient chimeric tissues. Mol. Cell. Bio. 27(6):2283 - 93
  3. Moberg K, Starz M, Lees JA (1996) E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry. Mol. Cell. Bio. 16(4): 1436 - 49

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